不同来源间充质基质细胞对急性脑缺血大鼠海马CA1区形态的影响

S. Konovalov, V. Moroz, O. Deryabina, P. Klymenko, A. Tochylovsky, V. Kordium
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引用次数: 1

摘要

乌克兰每年约有15万人中风,超过10万人死于中风和其他大脑循环系统疾病。到目前为止,已经获得了利用间充质基质细胞(MSC)治疗神经功能障碍的有希望的实验数据。目的:根据形态计量学数据,研究不同来源的间充质干细胞、华顿冻干细胞裂解物和胞磷胆碱对急性脑缺血模型大鼠海马CA1区破坏性变化动力学的影响。材料和方法。使用4个月大的雄性Wistar大鼠进行实验,对其进行短暂的双侧颈内动脉20分钟缺血再灌注(IR)。建模后,将沃顿果冻衍生的MSC、人和大鼠脂肪衍生的MSC以106个细胞/只的剂量静脉注射给动物。其他组以106个细胞/动物的剂量静脉注射大鼠胎儿成纤维细胞,并以0.2mL/动物的剂量注射来自沃顿脐带MSC的裂解物。对照动物注射0.2mL生理盐水。最后一组大鼠接受单剂量的参考药物胞磷胆碱,剂量为250mg/kg。在第7天和第14天,在海马CA1区计数每1mm2脑切片的神经元细胞核总数,并测定完整的神经元细胞核数与有变化的细胞核数(核破裂和核固缩)的比率。后果骨髓间充质干细胞、沃顿果冻来源的骨髓间充细胞裂解物或胞磷胆碱的移植有助于海马CA1区细胞核数量的增加,并且未发生病理变化的细胞核数量也增加。移植华顿果冻来源的骨髓间充质干细胞具有最积极的效果。该组动物的海马CA1区每1mm2的神经元细胞核数接近假手术动物组的细胞核数。同时,没有发生病理变化的细胞核数量显著超过有破坏迹象的细胞核数量。结论在给予各种类型的所研究的间充质基质细胞、裂解物或胞磷胆碱后的缺血性脑损伤建模过程中,在所有实验组大鼠中观察到没有病理变化迹象的神经元数量显著增加。在给予沃顿果冻衍生的MSC后,在海马CA1区获得了最积极的结果。
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The effect of mesenchymal stromal cells of various origins on morphology of hippocampal CA1 area of rats with acute cerebral ischemia
Every year, about 150,000 strokes occur in Ukraine, and more than 100,000 people die from the consequences of stroke and other circulatory disorders in the brain. So far, promising experimental data on the treatment of neurological dysfunction using mesenchymal stromal cells (MSCs) have been obtained. Purpose: to characterize the impact of MSCs of various origins, lysate of Wharton’s jelly-derived MSCs and citicoline on the dynamics of destructive changes in the hippocampal CA1 area of rats with model of acute cerebral ischemia according to morphometric data. Materials and methods. An experiment was performed using 4-month-old male Wistar rats, which were subjected to transient bilateral 20-minute ischemia-reperfusion (IR) of the internal carotid arteries. After modeling, the animals were injected intravenously with Wharton’s jelly-derived MSCs, human and rat adipose-derived MSCs at a dose 106 cells/animal. Other groups were intravenously injected with rat fetal fibroblasts at a dose of 106 cells/animal and lysate from Wharton’s umbilical cord MSCs at a dose of 0.2 mL/animal. Control animals were injected with 0.2 mL of saline. The last group of rats received a single dose of the reference drug citicoline at a dose of 250 mg/kg. On the 7th and 14th day, the total number of neuron nuclei per 1 mm2 brain section was counted in the hippocampal CA1 area, and the ratio of the number of intact neuron nuclei and nuclei with changes (karyorrhexis and karyopyknosis) was determined. Results. The transplantation of MSCs, lysate of Wharton’s jelly-derived MSCs, or citicoline contributed to a greater value of the number of nuclei in the hippocampal CA1 area, and the number of nuclei that did not undergo pathological changes also increased. The transplantation of Wharton’s jelly-derived MSCs had the most positive effect. The number of neuron nuclei per 1 mm2 in the hippocampal CA1 area in this group of animals approached the number of nuclei in the group of sham-operated animals. At the same time, the number of nuclei that did not undergo pathological changes significantly exceeded the number of nuclei with signs of destruction. Conclusion. A significant increase in the number of neurons without signs of pathological changes was observed in all experimental groups of rats during the modeling of ischemic brain injury after the administration of various types of studied mesenchymal stromal cells, lysate or citicoline. The most positive result in the hippocampal CA1 area was achieved after the administration of Wharton’s jelly-derived MSCs.
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来源期刊
Cell and Organ Transplantology
Cell and Organ Transplantology Medicine-Transplantation
CiteScore
0.40
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发文量
8
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