从蝌蚪尾尖再生组织制备活体内有丝分裂染色体的方法

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2022-04-14 DOI:10.25225/jvb.22010

E. Pustovalova, A. Fedorova, Dmytro Shabanov

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引用次数: 0

摘要

摘要我们提出了一种改进和更新的方案，从Pelophilax蝌蚪尾尖的再生组织中获得有丝分裂染色体。再生组织的染色体制备得到高质量、干净的载玻片，适合进一步染色和研究。蝌蚪仍然活着，承受的痛苦最小，可以长大成人接受进一步的调查。该方法可用于其他无尾类，也可用于其他具有再生组织能力的物种。

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Methodology for intravital mitotic chromosome preparation from regenerated tissue derived from the tail tips of tadpoles

Abstract. We propose a modified and updated protocol to obtain mitotic chromosomes from the regenerated tissue of Pelophylax tadpole tail tips. Chromosomal preparations from regenerated tissue results in high-quality and clean slides suitable for further staining and study. Tadpoles remain alive, undergo minimum suffering, and can be grown to adulthood for further investigation. The method could be used for other groups of Anura and modified for other species with the ability to regenerate their tissues.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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