alceoides对宫颈癌细胞的体外细胞毒活性

IF 0.7 Q4 PHARMACOLOGY & PHARMACY Journal of Reports in Pharmaceutical Sciences Pub Date : 2020-01-01 DOI:10.4103/jrptps.JRPTPS_65_19
Masoud Sadeghi Dinani, Samin Malakooti, Vajihe Akbari
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引用次数: 1

摘要

背景:牙垢具有多种药理活性,包括抗炎、镇咳、抗溃疡、免疫调节、抗菌、抗疟、抗氧化和抗癌活性。目的:本工作的目的是评估该属不同级分的嗜碱Verbascus alceoides的细胞毒性。材料和方法:从科尔德斯坦省Doveiseh地区采集该植物的地上部分。使用四步萃取法提取植物,增加溶剂极性(即己烷、二氯甲烷、氯仿-甲醇[9:1]和甲醇)。甲醇提取物最终在水和丁醇之间分离。然后对己烷、二氯甲烷、三氯甲烷-甲醇、丁醇和水性隔板进行细胞毒性评估。显示出最强大的细胞毒性作用,通过中效液相色谱法进一步分馏丁醇分配,并将类似的洗脱液合并以制备五个最终的丁醇级分,命名为A–E。结果:用3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2H-四唑溴化法评价了这些组分对人宫颈上皮样癌(HeLa)和人脐静脉内皮细胞(HUVEC)的体外细胞毒性。组分D、E和A显示出对细胞增殖的显著和剂量依赖性抑制(半数最大抑制浓度[IC50]分别为30、39.8和188.6µg/mL)。根据初步的薄层色谱分析,这些细胞毒性作用可能主要是由于皂苷和类黄酮化合物的存在。结论:今后的研究旨在分离和纯化活性成分,并研究其对更多不同类型癌症细胞的作用。
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In vitro cytotoxic activity of Verbascum alceoides against cervix carcinoma cells
Background: Verbascum species showed various pharmacological activities including anti-inflammatory, antitussive, antiulcerogenic, immunomodulatory, antimicrobial, antimalarial, antioxidant, and anticancer activities. Objectives: The aim of this work was to evaluate cytotoxicity of different fractions of Verbascum alceoides, which belongs to this genus. Materials and Methods: Aerial parts of this plant were collected from Doveiseh area in Kordestan province. The plant was extracted using a four-step extraction method with increasing solvent polarity (i.e., hexane, dichloromethane, chloroform-methanol [9:1], and methanol). The methanol extract was finally separated between water and butanol. Hexane, dichloromethane, chloroform-methanol, butanol, and aqueous partitions were then subjected to cytotoxicity evaluation. Showing the most potent cytotoxic effects, the butanolic partition was further fractionated by medium-performance liquid chromatography and similar eluates were pooled to prepare five final butanolic fractions, named A–E. Results: In vitro cytotoxicity of these fractions against human cervical epithelioid carcinoma (HeLa) and human umbilical vein endothelial cell (HUVEC) was evaluated using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. Fractions D, E, and A showed a significant and dose-dependent inhibition of cell proliferation (half maximal inhibitory concentration [IC50] of 30, 39.8, and 188.6 µg/mL, respectively). According to the preliminary thin-layer chromatography analysis, these cytotoxic effects may be mainly due to presence of saponin and flavonoid compounds. Conclusion: Future studies will be aimed to isolate and purify active constituents and investigate the effect of them on more different kinds of cancer cells.
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来源期刊
Journal of Reports in Pharmaceutical Sciences
Journal of Reports in Pharmaceutical Sciences Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)
CiteScore
1.40
自引率
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0
期刊介绍: The Journal of Reports in Pharmaceutical Sciences(JRPS) is a biannually peer-reviewed multi-disciplinary pharmaceutical publication to serve as a means for scientific information exchange in the international pharmaceutical forum. It accepts novel findings that contribute to advancement of scientific knowledge in pharmaceutical fields that not published or under consideration for publication anywhere else for publication in JRPS as original research article. all aspects of pharmaceutical sciences consist of medicinal chemistry, molecular modeling, drug design, pharmaceutics, biopharmacy, pharmaceutical nanotechnology, pharmacognosy, natural products, pharmaceutical biotechnology, pharmacology, toxicology and clinical pharmacy.
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