不同人工甜味剂对蛋白质糖基化的影响

Q3 Agricultural and Biological Sciences Acta Biologica Szegediensis Pub Date : 2023-05-27 DOI:10.14232/abs.2022.2.132-138
D. Kumar, Ahmad Ali
{"title":"不同人工甜味剂对蛋白质糖基化的影响","authors":"D. Kumar, Ahmad Ali","doi":"10.14232/abs.2022.2.132-138","DOIUrl":null,"url":null,"abstract":"Glycation refers to the non-enzymatic molecular interaction between carbonyl group of sugars and amino groups of macromolecules viz. proteins, DNA, and lipids leading to the generation of Schiff’s base, Amadori products, and finally converted to deleterious advanced glycation end-products (AGEs). Several diseases such as neurodegenerative or mental disorders, cardiovascular complications, as well as diabetes, and its related complications show glycated product involvement. Hyperglycemia and diabetes are the main diseases in which AGEs formation and its accumulation are enhanced and cause secondary complications. This study was performed to investigate the antiglycation and anti-aggregation potential of Food and Drug Administration-approved artificial sweeteners. The in vitro glycation system (BSA and glucose) was incubated along with artificial sweeteners viz acesulfame potassium, saccharin sodium, sucralose, aspartame, and neotame for 35 days at 37 °C. The conventional analytical methods such as browning, NBT assay, DNPH assay, and assessment of fluorescent AGEs were carried out spectroscopically to check the amount of glycation products. The presence of the mentioned artificial sweeteners in the glycation system showed inhibition of carbonyl content, total AGEs generation, and aggregation of β-amyloid structures. On day 35, acesulfame potassium reduced carbonyl content by 62.63 ± 0.91%, total AGEs generation by 49.39 ± 0.82%, and β-amyloid aggregation observed by Thioflavin-T assay by 43.45 ± 1.14%. The tested artificial sweeteners exhibited potential antiglycation and anti-aggregation activity in vitro in protein, BSA. They may be used as a therapeutic agent for the management of diabetes and its complications.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of different artificial sweeteners on protein glycation\",\"authors\":\"D. Kumar, Ahmad Ali\",\"doi\":\"10.14232/abs.2022.2.132-138\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Glycation refers to the non-enzymatic molecular interaction between carbonyl group of sugars and amino groups of macromolecules viz. proteins, DNA, and lipids leading to the generation of Schiff’s base, Amadori products, and finally converted to deleterious advanced glycation end-products (AGEs). Several diseases such as neurodegenerative or mental disorders, cardiovascular complications, as well as diabetes, and its related complications show glycated product involvement. Hyperglycemia and diabetes are the main diseases in which AGEs formation and its accumulation are enhanced and cause secondary complications. This study was performed to investigate the antiglycation and anti-aggregation potential of Food and Drug Administration-approved artificial sweeteners. The in vitro glycation system (BSA and glucose) was incubated along with artificial sweeteners viz acesulfame potassium, saccharin sodium, sucralose, aspartame, and neotame for 35 days at 37 °C. The conventional analytical methods such as browning, NBT assay, DNPH assay, and assessment of fluorescent AGEs were carried out spectroscopically to check the amount of glycation products. The presence of the mentioned artificial sweeteners in the glycation system showed inhibition of carbonyl content, total AGEs generation, and aggregation of β-amyloid structures. On day 35, acesulfame potassium reduced carbonyl content by 62.63 ± 0.91%, total AGEs generation by 49.39 ± 0.82%, and β-amyloid aggregation observed by Thioflavin-T assay by 43.45 ± 1.14%. The tested artificial sweeteners exhibited potential antiglycation and anti-aggregation activity in vitro in protein, BSA. They may be used as a therapeutic agent for the management of diabetes and its complications.\",\"PeriodicalId\":34918,\"journal\":{\"name\":\"Acta Biologica Szegediensis\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-05-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Biologica Szegediensis\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.14232/abs.2022.2.132-138\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Biologica Szegediensis","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.14232/abs.2022.2.132-138","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0

摘要

糖基化是指糖的羰基和大分子(即蛋白质、DNA和脂质)的氨基之间的非酶分子相互作用,导致希夫碱Amadori产物的产生,并最终转化为有害的晚期糖基化终产物(AGEs)。一些疾病,如神经退行性或精神障碍、心血管并发症、糖尿病及其相关并发症,都显示出糖化产物的参与。高血糖和糖尿病是AGEs形成和积累增强并引起继发并发症的主要疾病。本研究旨在研究美国食品药品监督管理局批准的人工甜味剂的抗糖化和抗聚集潜力。体外糖化系统(BSA和葡萄糖)与人工甜味剂安赛蜜钾、糖精钠、三氯蔗糖、阿斯巴甜和新甜在37°C下孵育35天。常规分析方法,如褐变、NBT测定、DNPH测定和荧光AGEs的评估,用光谱法检测糖化产物的量。上述人工甜味剂在糖化系统中的存在显示出对羰基含量、总AGEs生成和β-淀粉样蛋白结构聚集的抑制作用。在第35天,安赛蜜钾使羰基含量降低了62.63±0.91%,总AGEs生成减少了49.39±0.82%,硫黄素-T法观察到的β-淀粉样蛋白聚集减少了43.45±1.14%。所测试的人工甜味剂在蛋白质BSA中表现出潜在的抗糖基化和抗聚集活性。它们可以用作糖尿病及其并发症的治疗剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Effect of different artificial sweeteners on protein glycation
Glycation refers to the non-enzymatic molecular interaction between carbonyl group of sugars and amino groups of macromolecules viz. proteins, DNA, and lipids leading to the generation of Schiff’s base, Amadori products, and finally converted to deleterious advanced glycation end-products (AGEs). Several diseases such as neurodegenerative or mental disorders, cardiovascular complications, as well as diabetes, and its related complications show glycated product involvement. Hyperglycemia and diabetes are the main diseases in which AGEs formation and its accumulation are enhanced and cause secondary complications. This study was performed to investigate the antiglycation and anti-aggregation potential of Food and Drug Administration-approved artificial sweeteners. The in vitro glycation system (BSA and glucose) was incubated along with artificial sweeteners viz acesulfame potassium, saccharin sodium, sucralose, aspartame, and neotame for 35 days at 37 °C. The conventional analytical methods such as browning, NBT assay, DNPH assay, and assessment of fluorescent AGEs were carried out spectroscopically to check the amount of glycation products. The presence of the mentioned artificial sweeteners in the glycation system showed inhibition of carbonyl content, total AGEs generation, and aggregation of β-amyloid structures. On day 35, acesulfame potassium reduced carbonyl content by 62.63 ± 0.91%, total AGEs generation by 49.39 ± 0.82%, and β-amyloid aggregation observed by Thioflavin-T assay by 43.45 ± 1.14%. The tested artificial sweeteners exhibited potential antiglycation and anti-aggregation activity in vitro in protein, BSA. They may be used as a therapeutic agent for the management of diabetes and its complications.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Acta Biologica Szegediensis
Acta Biologica Szegediensis Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
1.00
自引率
0.00%
发文量
14
期刊介绍: Acta Biologica Szegediensis (ISSN 1588-385X print form; ISSN 1588-4082 online form), a member of the Acta Universitatis Szegediensis family of scientific journals (ISSN 0563-0592), is published yearly by the University of Szeged. Acta Biologica Szegediensis covers the growth areas of modern biology and publishes original research articles and reviews, involving, but not restricted to, the fields of anatomy, embryology and histology, anthropology, biochemistry, biophysics, biotechnology, botany and plant physiology, all areas of clinical sciences, conservation biology, ecology, genetics, microbiology, molecular biology, neurosciences, paleontology, pharmacology, physiology and pathophysiology, and zoology.
期刊最新文献
The pangenome structure of human pathogen Mycobacterium kansasii Effect of water deprivation for three consecutive days on the proportions of androgen receptor-immunolabeled supraoptic nucleus magnoneurons in Wistar rats Investigating crossed-symmetry pattern in children from the past A longitudinal study on the prevalence of iron deficiency anemia in the multiethnic communities of Jhargram, West Bengal Comparative mathematical analysis of economically important ampelographic indicators in white wine vine varieties (Vitis vinifera L.)
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1