Ulf Jensen-Kondering , Liang Shu , Ruwen Böhm , Olav Jansen , Ulrich Katscher
{"title":"3特斯拉的体内实验研究:脑卒中大鼠模型电特性断层扫描的可行性","authors":"Ulf Jensen-Kondering , Liang Shu , Ruwen Böhm , Olav Jansen , Ulrich Katscher","doi":"10.1016/j.phmed.2019.100024","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Electrical Properties Tomography (EPT) is a new sequence which delivers information on tissue electrical conductivity. It has been mostly used for tumor imaging. Ischemic stroke is another promising application. The aim of this study was to demonstrate the feasibility of EPT in a rodent model of stroke.</p></div><div><h3>Methods</h3><p>Wistar rats with and without temporary middle cerebral occlusion (MCAo) were examined in a 3T scanner. EPT was performed using a Steady-State Free-Precession (SSFP) sequence. From the transceive phase ɸ of these SSFP scans, conductivity σ was estimated by the equation σ = Δɸ/(2μ<sub>0</sub>ω) with Δ the Laplacian operator, μ<sub>0</sub> the magnetic permeability, and ω the Larmor frequency. Subsequently, a median filter was applied.</p></div><div><h3>Results</h3><p>Healthy cortical grey matter, white matter and cerebrospinal fluid showed significantly different conductivity (0.83 ± 0.14 S/m, 0.63 ± 0.06 S/m, 2.33 ± 0.49 S/m, p < 0.05). Infarcted tissue exhibited increased conductivity (1.937 ± 1.347 vs. 0.782 ± 0.429 S/m, p < 0.05).</p></div><div><h3>Conclusion</h3><p>EPT is feasible in a rodent model of stroke. Infarcted tissue after MCAo exhibited increased conductivity. Further in-vivo experiments with examination of the influence of reperfusion status and temporal evolution of the infarcted areas should be conducted.</p></div>","PeriodicalId":37787,"journal":{"name":"Physics in Medicine","volume":"9 ","pages":"Article 100024"},"PeriodicalIF":0.0000,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.phmed.2019.100024","citationCount":"5","resultStr":"{\"title\":\"In-vivo pilot study at 3 Tesla: Feasibility of Electric Properties Tomography in a rat model of stroke\",\"authors\":\"Ulf Jensen-Kondering , Liang Shu , Ruwen Böhm , Olav Jansen , Ulrich Katscher\",\"doi\":\"10.1016/j.phmed.2019.100024\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Electrical Properties Tomography (EPT) is a new sequence which delivers information on tissue electrical conductivity. It has been mostly used for tumor imaging. Ischemic stroke is another promising application. The aim of this study was to demonstrate the feasibility of EPT in a rodent model of stroke.</p></div><div><h3>Methods</h3><p>Wistar rats with and without temporary middle cerebral occlusion (MCAo) were examined in a 3T scanner. EPT was performed using a Steady-State Free-Precession (SSFP) sequence. From the transceive phase ɸ of these SSFP scans, conductivity σ was estimated by the equation σ = Δɸ/(2μ<sub>0</sub>ω) with Δ the Laplacian operator, μ<sub>0</sub> the magnetic permeability, and ω the Larmor frequency. Subsequently, a median filter was applied.</p></div><div><h3>Results</h3><p>Healthy cortical grey matter, white matter and cerebrospinal fluid showed significantly different conductivity (0.83 ± 0.14 S/m, 0.63 ± 0.06 S/m, 2.33 ± 0.49 S/m, p < 0.05). Infarcted tissue exhibited increased conductivity (1.937 ± 1.347 vs. 0.782 ± 0.429 S/m, p < 0.05).</p></div><div><h3>Conclusion</h3><p>EPT is feasible in a rodent model of stroke. Infarcted tissue after MCAo exhibited increased conductivity. Further in-vivo experiments with examination of the influence of reperfusion status and temporal evolution of the infarcted areas should be conducted.</p></div>\",\"PeriodicalId\":37787,\"journal\":{\"name\":\"Physics in Medicine\",\"volume\":\"9 \",\"pages\":\"Article 100024\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.phmed.2019.100024\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Physics in Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2352451019300319\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Physics in Medicine","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2352451019300319","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 5
摘要
电性质层析成像(EPT)是一种提供组织电导率信息的新序列。它主要用于肿瘤成像。缺血性中风是另一个有前途的应用。本研究的目的是证明EPT在啮齿动物脑卒中模型中的可行性。方法采用3T扫描对有无暂时性大脑中闭塞(MCAo)的swistar大鼠进行观察。使用稳态自由进动(SSFP)序列进行EPT。根据SSFP扫描的收发相位，利用拉普拉斯算子Δ，磁导率μ0，拉莫尔频率ω，计算出电导率σ = Δ h /(2μ0ω)。随后，应用中值滤波器。结果健康脑皮层灰质、白质和脑脊液电导率分别为0.83±0.14 S/m、0.63±0.06 S/m、2.33±0.49 S/m, p <0.05)。梗死组织电导率升高(1.937±1.347 vs. 0.782±0.429 S/m);0.05)。结论ept在鼠类脑卒中模型中是可行的。MCAo后梗死组织的电导率增加。进一步的体内实验，以检查再灌注状态和梗塞区域的时间演变的影响。
In-vivo pilot study at 3 Tesla: Feasibility of Electric Properties Tomography in a rat model of stroke
Background
Electrical Properties Tomography (EPT) is a new sequence which delivers information on tissue electrical conductivity. It has been mostly used for tumor imaging. Ischemic stroke is another promising application. The aim of this study was to demonstrate the feasibility of EPT in a rodent model of stroke.
Methods
Wistar rats with and without temporary middle cerebral occlusion (MCAo) were examined in a 3T scanner. EPT was performed using a Steady-State Free-Precession (SSFP) sequence. From the transceive phase ɸ of these SSFP scans, conductivity σ was estimated by the equation σ = Δɸ/(2μ0ω) with Δ the Laplacian operator, μ0 the magnetic permeability, and ω the Larmor frequency. Subsequently, a median filter was applied.
Results
Healthy cortical grey matter, white matter and cerebrospinal fluid showed significantly different conductivity (0.83 ± 0.14 S/m, 0.63 ± 0.06 S/m, 2.33 ± 0.49 S/m, p < 0.05). Infarcted tissue exhibited increased conductivity (1.937 ± 1.347 vs. 0.782 ± 0.429 S/m, p < 0.05).
Conclusion
EPT is feasible in a rodent model of stroke. Infarcted tissue after MCAo exhibited increased conductivity. Further in-vivo experiments with examination of the influence of reperfusion status and temporal evolution of the infarcted areas should be conducted.
期刊介绍：
The scope of Physics in Medicine consists of the application of theoretical and practical physics to medicine, physiology and biology. Topics covered are: Physics of Imaging Ultrasonic imaging, Optical imaging, X-ray imaging, Fluorescence Physics of Electromagnetics Neural Engineering, Signal analysis in Medicine, Electromagnetics and the nerve system, Quantum Electronics Physics of Therapy Ultrasonic therapy, Vibrational medicine, Laser Physics Physics of Materials and Mechanics Physics of impact and injuries, Physics of proteins, Metamaterials, Nanoscience and Nanotechnology, Biomedical Materials, Physics of vascular and cerebrovascular diseases, Micromechanics and Micro engineering, Microfluidics in medicine, Mechanics of the human body, Rotary molecular motors, Biological physics, Physics of bio fabrication and regenerative medicine Physics of Instrumentation Engineering of instruments, Physical effects of the application of instruments, Measurement Science and Technology, Physics of micro-labs and bioanalytical sensor devices, Optical instrumentation, Ultrasound instruments Physics of Hearing and Seeing Acoustics and hearing, Physics of hearing aids, Optics and vision, Physics of vision aids Physics of Space Medicine Space physiology, Space medicine related Physics.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
