Preparation of Nanogold and binding of nisin to increase its effectiveness in inhibiting the positive and negative bacteria

Most pathogenic bacteria have the acquisition of antibiotic resistance. Therefore, continuous research to find a new antibiotic against pathogens is an urgent task to avoid the rapid spreading of diseases. The direction is to create nanomaterials instead of common antibiotics to inhibition some bacteria high effectively. The purpose of this study was to functionalize the gold nanoparticles (AuNPs) with nisin (N) and applied to a group of pathogenic bacteria to evaluate the effectiveness of them in killing the isolated bacteria, including Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Bacillus subtilis, Staphylococcus aureus .  The TEM study revealed the grain size of AuNPs ranging 1-14nm, whereas the grain size of AuNPs-N ranging 1-120nm. Also, we were measured the XRD of AuNPs and AuNPs-N to find out their structural properties. In addition to the evaluation of biologically active substances by FTIR system. The results through the biological activity showed that the nisin functionalized gold nanoparticles (AuNPs-N) exhibited perfect inhibitory activity against all the isolated bacteria.