金黄色葡萄球菌表面蛋白FnBPB与角膜粘连蛋白的相互作用涉及两个不同的,极强的键

IF 4.8 Q2 NANOSCIENCE & NANOTECHNOLOGY ACS Nanoscience Au Pub Date : 2022-10-18 DOI:10.1021/acsnanoscienceau.2c00036
Telmo O. Paiva, Albertus Viljoen, Thaina M. da Costa, Joan A. Geoghegan* and Yves F. Dufrêne*, 
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引用次数: 2

摘要

金黄色葡萄球菌与人类皮肤角质细胞的粘附在加剧特应性皮炎(AD)的严重程度中起着关键作用。病原体皮肤粘附是由称为粘附素的细菌细胞表面蛋白介导的,包括纤连蛋白结合蛋白B(FnBPB)。FnBPB与角膜内皮素(CDSN)结合,CDSN是一种暴露在AD患者角膜细胞上的糖蛋白。通过单分子实验,我们证明FnBPB与CDSN的结合依赖于复杂的双位点机制。这两个位点都形成了极强的结合,结合力分别为~1和~2.5nN,尽管解离速率比粘附素同源物的解离速率更快。FnBPB中这种先前未确定的两个结合位点的相互作用说明了其显著的多种粘附功能,并且具有生物学意义,因为高强度和短的结合寿命将有利于病原体有效的皮肤定植。
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Interaction of the Staphylococcus aureus Surface Protein FnBPB with Corneodesmosin Involves Two Distinct, Extremely Strong Bonds

Attachment of Staphylococcus aureus to human skin corneocyte cells plays a critical role in exacerbating the severity of atopic dermatitis (AD). Pathogen-skin adhesion is mediated by bacterial cell-surface proteins called adhesins, including fibronectin-binding protein B (FnBPB). FnBPB binds to corneodesmosin (CDSN), a glycoprotein exposed on AD patient corneocytes. Using single-molecule experiments, we demonstrate that CDSN binding by FnBPB relies on a sophisticated two-site mechanism. Both sites form extremely strong bonds with binding forces of ∼1 and ∼2.5 nN albeit with faster dissociation rates than those reported for homologues of the adhesin. This previously unidentified two-binding site interaction in FnBPB illustrates its remarkable variety of adhesive functions and is of biological significance as the high strength and short bond lifetime will favor efficient skin colonization by the pathogen.

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来源期刊
ACS Nanoscience Au
ACS Nanoscience Au 材料科学、纳米科学-
CiteScore
4.20
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0.00%
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0
期刊介绍: ACS Nanoscience Au is an open access journal that publishes original fundamental and applied research on nanoscience and nanotechnology research at the interfaces of chemistry biology medicine materials science physics and engineering.The journal publishes short letters comprehensive articles reviews and perspectives on all aspects of nanoscience and nanotechnology:synthesis assembly characterization theory modeling and simulation of nanostructures nanomaterials and nanoscale devicesdesign fabrication and applications of organic inorganic polymer hybrid and biological nanostructuresexperimental and theoretical studies of nanoscale chemical physical and biological phenomenamethods and tools for nanoscience and nanotechnologyself- and directed-assemblyzero- one- and two-dimensional materialsnanostructures and nano-engineered devices with advanced performancenanobiotechnologynanomedicine and nanotoxicologyACS Nanoscience Au also publishes original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials engineering physics bioscience and chemistry into important applications of nanomaterials.
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