Xiurong Kang , Qinghu Wang , Suriguga Ao , Moxiyele , Wenlin Bao , Chaoliang Zhao
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The flow rate was 1.0 mL/min, the column temperature was 40 °C, the injection volume was 10 μL, and monitored by absorbance at 285 nm for compounds <strong>1</strong>–<strong>10</strong>, <strong>12</strong> and 225 nm for compounds <strong>11</strong>, <strong>13</strong>–<strong>16</strong>.</p></div><div><h3>Results</h3><p>Under the selected experimental chromatographic conditions, compounds <strong>1</strong>–<strong>16</strong> showed good linearity (<em>r</em> > 0.9993) in a wide concentration range. Their average recoveries were 99.50%, 95.38%, 97.75%, 96.00%, 98.20%, 97.50%, 95.50%, 99.33%, 96.75%, 96.50%, 98.50%, 97.83%, 99.20%, 95.33%, 97.33% and 96.30%, respectively, and the RSD were 1.99%, 1.81%, 1.63%, 1.98%, 1.67%, 1.92%, 1.74%, 1.67%, 1.90%, 1.72%, 1.88%, 1.83%, 1.79%, 1.76%, 1.81% and 1.96%, respectively.</p></div><div><h3>Conclusion</h3><p>Based on the results of the HPLC analysis, it was concluded that <em>p</em>-hydroxycinnamic acid (<strong>1</strong>), <em>O</em>-hydroxycinnamic acid (<strong>2</strong>), coniferyl alcohol (<strong>5</strong>), 5,4′-dihydroxy-7,3′-dimethoxyflavanone (<strong>8</strong>), 5,4′-dihydroxy-7-methoxyflavanone (<strong>9</strong>), 5-hydroxy-7,4′-dimethoxyflavanone (<strong>12</strong>), dehydrofalcarindiol (<strong>13</strong>), arteordoyn A (<strong>14</strong>), dehydrofalcarinol (<strong>15</strong>) and capillarin (<strong>16</strong>) are best suited for the role of quality indicators of <em>A. ordosica</em> grown in different ecological environments.</p></div>","PeriodicalId":9916,"journal":{"name":"Chinese Herbal Medicines","volume":null,"pages":null},"PeriodicalIF":4.7000,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"HPLC analysis of 16 compounds from Artemisia ordosica\",\"authors\":\"Xiurong Kang , Qinghu Wang , Suriguga Ao , Moxiyele , Wenlin Bao , Chaoliang Zhao\",\"doi\":\"10.1016/j.chmed.2022.08.009\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><p>To establish a high-performance liquid chromatographic method (HPLC) for the simultaneous determination of 16 compounds from <em>Artemisia ordosica</em>.</p></div><div><h3>Methods</h3><p>HPLC was used to analyze 16 quality indicators of <em>A. ordosica</em>. The HPLC conditions were as follows: Agilent Eclipse Plus C<sub>18</sub> column (250 mm × 4.6 mm, 5 μm) with acetonitrile (A)-water (B) as mobile phase, gradient elution: 0–10 min, 75%−65% B; 10–30 min, 65%−35% B; and finally 30–40 min, 35%−15% B. The flow rate was 1.0 mL/min, the column temperature was 40 °C, the injection volume was 10 μL, and monitored by absorbance at 285 nm for compounds <strong>1</strong>–<strong>10</strong>, <strong>12</strong> and 225 nm for compounds <strong>11</strong>, <strong>13</strong>–<strong>16</strong>.</p></div><div><h3>Results</h3><p>Under the selected experimental chromatographic conditions, compounds <strong>1</strong>–<strong>16</strong> showed good linearity (<em>r</em> > 0.9993) in a wide concentration range. Their average recoveries were 99.50%, 95.38%, 97.75%, 96.00%, 98.20%, 97.50%, 95.50%, 99.33%, 96.75%, 96.50%, 98.50%, 97.83%, 99.20%, 95.33%, 97.33% and 96.30%, respectively, and the RSD were 1.99%, 1.81%, 1.63%, 1.98%, 1.67%, 1.92%, 1.74%, 1.67%, 1.90%, 1.72%, 1.88%, 1.83%, 1.79%, 1.76%, 1.81% and 1.96%, respectively.</p></div><div><h3>Conclusion</h3><p>Based on the results of the HPLC analysis, it was concluded that <em>p</em>-hydroxycinnamic acid (<strong>1</strong>), <em>O</em>-hydroxycinnamic acid (<strong>2</strong>), coniferyl alcohol (<strong>5</strong>), 5,4′-dihydroxy-7,3′-dimethoxyflavanone (<strong>8</strong>), 5,4′-dihydroxy-7-methoxyflavanone (<strong>9</strong>), 5-hydroxy-7,4′-dimethoxyflavanone (<strong>12</strong>), dehydrofalcarindiol (<strong>13</strong>), arteordoyn A (<strong>14</strong>), dehydrofalcarinol (<strong>15</strong>) and capillarin (<strong>16</strong>) are best suited for the role of quality indicators of <em>A. ordosica</em> grown in different ecological environments.</p></div>\",\"PeriodicalId\":9916,\"journal\":{\"name\":\"Chinese Herbal Medicines\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.7000,\"publicationDate\":\"2023-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chinese Herbal Medicines\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1674638423000084\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MEDICINAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chinese Herbal Medicines","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1674638423000084","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
引用次数: 1
摘要
目的建立同时测定油蒿中16种化合物含量的高效液相色谱法。方法采用高效液相色谱法对16项油姜药材的质量指标进行分析。高效液相色谱条件为:Agilent Eclipse Plus C18柱(250 mm×4.6 mm,5μm),流动相为乙腈(A)-水(B),梯度洗脱:0–10 min,75%−65%B;10-30分钟,65%−35%B;最后是30–40分钟,35%-15%B。流速为1.0 mL/min,柱温为40°C,进样体积为10μL,通过化合物1–10、12和11、13–16在285 nm处的吸光度进行监测。结果在选定的实验色谱条件下,化合物1–16在宽浓度范围内显示出良好的线性(r>;0.9993)。平均回收率分别为99.50%、95.38%、97.75%、96.00%、98.20%、97.50%、95.50%、99.33%、96.75%、96.50%、98.50%、97.83%、99.20%、95.33%、97.33%和96.30%,RSD分别为1.99%、1.81%、1.63%、1.98%、1.67%、1.92%、1.74%、1.67%,1.90%、1.72%、1.88%、1.83%、1.79%、1.76%、1.81%和1.96%。结论高效液相色谱分析结果表明,对羟基肉桂酸(1)、O-羟基肉桂酸,脱氢镰刀菌烯醇(15)和贝母素(16)最适合作为不同生态环境中生长的油姜的质量指标。
HPLC analysis of 16 compounds from Artemisia ordosica
Objective
To establish a high-performance liquid chromatographic method (HPLC) for the simultaneous determination of 16 compounds from Artemisia ordosica.
Methods
HPLC was used to analyze 16 quality indicators of A. ordosica. The HPLC conditions were as follows: Agilent Eclipse Plus C18 column (250 mm × 4.6 mm, 5 μm) with acetonitrile (A)-water (B) as mobile phase, gradient elution: 0–10 min, 75%−65% B; 10–30 min, 65%−35% B; and finally 30–40 min, 35%−15% B. The flow rate was 1.0 mL/min, the column temperature was 40 °C, the injection volume was 10 μL, and monitored by absorbance at 285 nm for compounds 1–10, 12 and 225 nm for compounds 11, 13–16.
Results
Under the selected experimental chromatographic conditions, compounds 1–16 showed good linearity (r > 0.9993) in a wide concentration range. Their average recoveries were 99.50%, 95.38%, 97.75%, 96.00%, 98.20%, 97.50%, 95.50%, 99.33%, 96.75%, 96.50%, 98.50%, 97.83%, 99.20%, 95.33%, 97.33% and 96.30%, respectively, and the RSD were 1.99%, 1.81%, 1.63%, 1.98%, 1.67%, 1.92%, 1.74%, 1.67%, 1.90%, 1.72%, 1.88%, 1.83%, 1.79%, 1.76%, 1.81% and 1.96%, respectively.
Conclusion
Based on the results of the HPLC analysis, it was concluded that p-hydroxycinnamic acid (1), O-hydroxycinnamic acid (2), coniferyl alcohol (5), 5,4′-dihydroxy-7,3′-dimethoxyflavanone (8), 5,4′-dihydroxy-7-methoxyflavanone (9), 5-hydroxy-7,4′-dimethoxyflavanone (12), dehydrofalcarindiol (13), arteordoyn A (14), dehydrofalcarinol (15) and capillarin (16) are best suited for the role of quality indicators of A. ordosica grown in different ecological environments.