{"title":"构建组织:牙龈和脂肪来源的间充质细胞球体在3D挤压生物打印后的生存能力和功能","authors":"Polina Bikmulina , Nastasia Kosheleva , Yuri Efremov , Alesia Bakulina , Anastasia Kuryanova , Nadezhda Aksenova , Boris Shavkuta , Svetlana Kotova , Anastasia Shpichka , Peter Timashev","doi":"10.1016/j.bprint.2023.e00279","DOIUrl":null,"url":null,"abstract":"<div><p>While being the most extensively used cell type for spheroid-based 3D extrusion bioprinting, mesenchymal stromal cells<span> (MSCs) provide a wide spectrum of biological properties depending on their origin. Understanding the specifics of each heterogeneous MSCs population subgroup would allow one to increase the survivability<span> and functionality of the constructed tissue analogues. To answer this need, this study assessed the survivability, metabolic activity<span>, proliferation, sprouting, migration, and differentiation capacity of MSCs spheroids depending on the cell source (adipose tissue, AT-MSCs/gingiva, G-MSCs) and on the tissue construct's geometry (bioprinted/manually mixed). This study has demonstrated that the cell origin defines the dynamics of spheroid reactivation, resulting in a varying construct's morphology after a 14-days-long cultivation period. AT-MSCs migrate in the hydrogel faster, forming clusters of wide and short sprouts. G-MSCs, oppositely, produce thin, long, and branched sprouts. Hence, AT-MSCs can quickly populate the hydrogel volume, achieving a high cell density, while G-MSCs can cover larger areas, but with a more sprout-like phenotype.</span></span></span></p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Building a tissue: gingiva- and adipose-derived mesenchymal cell spheroids’ survivability and functionality after 3D extrusion bioprinting\",\"authors\":\"Polina Bikmulina , Nastasia Kosheleva , Yuri Efremov , Alesia Bakulina , Anastasia Kuryanova , Nadezhda Aksenova , Boris Shavkuta , Svetlana Kotova , Anastasia Shpichka , Peter Timashev\",\"doi\":\"10.1016/j.bprint.2023.e00279\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>While being the most extensively used cell type for spheroid-based 3D extrusion bioprinting, mesenchymal stromal cells<span> (MSCs) provide a wide spectrum of biological properties depending on their origin. Understanding the specifics of each heterogeneous MSCs population subgroup would allow one to increase the survivability<span> and functionality of the constructed tissue analogues. To answer this need, this study assessed the survivability, metabolic activity<span>, proliferation, sprouting, migration, and differentiation capacity of MSCs spheroids depending on the cell source (adipose tissue, AT-MSCs/gingiva, G-MSCs) and on the tissue construct's geometry (bioprinted/manually mixed). This study has demonstrated that the cell origin defines the dynamics of spheroid reactivation, resulting in a varying construct's morphology after a 14-days-long cultivation period. AT-MSCs migrate in the hydrogel faster, forming clusters of wide and short sprouts. G-MSCs, oppositely, produce thin, long, and branched sprouts. Hence, AT-MSCs can quickly populate the hydrogel volume, achieving a high cell density, while G-MSCs can cover larger areas, but with a more sprout-like phenotype.</span></span></span></p></div>\",\"PeriodicalId\":37770,\"journal\":{\"name\":\"Bioprinting\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioprinting\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2405886623000222\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Computer Science\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioprinting","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405886623000222","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Computer Science","Score":null,"Total":0}
Building a tissue: gingiva- and adipose-derived mesenchymal cell spheroids’ survivability and functionality after 3D extrusion bioprinting
While being the most extensively used cell type for spheroid-based 3D extrusion bioprinting, mesenchymal stromal cells (MSCs) provide a wide spectrum of biological properties depending on their origin. Understanding the specifics of each heterogeneous MSCs population subgroup would allow one to increase the survivability and functionality of the constructed tissue analogues. To answer this need, this study assessed the survivability, metabolic activity, proliferation, sprouting, migration, and differentiation capacity of MSCs spheroids depending on the cell source (adipose tissue, AT-MSCs/gingiva, G-MSCs) and on the tissue construct's geometry (bioprinted/manually mixed). This study has demonstrated that the cell origin defines the dynamics of spheroid reactivation, resulting in a varying construct's morphology after a 14-days-long cultivation period. AT-MSCs migrate in the hydrogel faster, forming clusters of wide and short sprouts. G-MSCs, oppositely, produce thin, long, and branched sprouts. Hence, AT-MSCs can quickly populate the hydrogel volume, achieving a high cell density, while G-MSCs can cover larger areas, but with a more sprout-like phenotype.
期刊介绍:
Bioprinting is a broad-spectrum, multidisciplinary journal that covers all aspects of 3D fabrication technology involving biological tissues, organs and cells for medical and biotechnology applications. Topics covered include nanomaterials, biomaterials, scaffolds, 3D printing technology, imaging and CAD/CAM software and hardware, post-printing bioreactor maturation, cell and biological factor patterning, biofabrication, tissue engineering and other applications of 3D bioprinting technology. Bioprinting publishes research reports describing novel results with high clinical significance in all areas of 3D bioprinting research. Bioprinting issues contain a wide variety of review and analysis articles covering topics relevant to 3D bioprinting ranging from basic biological, material and technical advances to pre-clinical and clinical applications of 3D bioprinting.