巨噬细胞分泌CD5L在脂质代谢诱导炎症损伤的动脉粥样硬化进展中的作用

IF 2.9 4区 医学 Q3 IMMUNOLOGY Archivum Immunologiae et Therapiae Experimentalis Pub Date : 2022-03-06 DOI:10.1007/s00005-022-00643-y
Liang Wang, Lijuan Liu, Wei Qian, Zeqi Zheng
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引用次数: 3

摘要

探讨巨噬细胞分泌外泌体蛋白CD5L促进动脉粥样硬化进展的分子机制。收集动脉粥样硬化(AS)患者20例,正常人20例。透射电镜观察外泌体的形态特征,Western blot检测外泌体的标记蛋白CD63和CD81。ELISA法检测各组血上清中炎症因子的分泌情况。采用transwell建立动脉粥样硬化细胞模型,分为三组:第一组采用外泌体抑制剂GW4869,第二组注射CD5L蛋白,第三组为模型对照组。透射电镜观察外泌体的形态特征,Western blot检测外泌体的标记蛋白CD63和CD81。ELISA法检测各组细胞中TNF-α、IL-1β、IL-6、IL-13、IL-17A、IL-31水平。免疫荧光法分析IL-17RA在血管平滑肌细胞中的表达及分布。AS组CD63、CD81、CD5L蛋白较健康组高表达。细胞检测结果显示,AS组CD63、CD81、CD5L蛋白水平明显高于正常组。免疫荧光显示,AS模型组细胞膜IL-17RA表达水平最高,GW4869组和CD5L组IL-17RA表达降低。AS中炎性因子的表达明显高于GW4869组和CD5L组。巨噬细胞分泌的外泌体蛋白CD5L以脂质代谢诱导的血管平滑肌细胞炎症损伤为基础,促进动脉粥样硬化的发展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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CD5L Secreted by Macrophage on Atherosclerosis Progression Based on Lipid Metabolism Induced Inflammatory Damage

To explore the molecular mechanism of exosomal protein CD5L secreted by macrophage to promote the progression of atherosclerosis. Twenty cases of patients with atherosclerosis (AS) and 20 cases of healthy subjects were collected. Morphological properties of exosomes were identified by transmission electron microscopy, and the marker proteins CD63 and CD81 of exosomes were measured by Western blot. The secretion of inflammatory factors in the blood supernatant were analyzed by ELISA. Atherosclerosis cell models were established by transwell and separated into three groups: first group was treated with exosome inhibitor (GW4869), second group was injected with CD5L protein and third group was model control. Morphological properties of exosomes were identified by transmission electron microscopy, and the marker proteins CD63 and CD81 of exosomes were measured by Western blot. The levels of TNF-α, IL-1β, IL-6, IL-13, IL-17A, IL-31 in the cells were analyzed by ELISA. Analysis of the expression and distribution of IL-17RA in vascular smooth muscle cells by immunofluorescence. The proteins of CD63, CD81, CD5L were high expressed in AS group compared to healthy subject group. Cell test results showed that protein levels of CD63, CD81, CD5L in AS group were much higher than that in normal group. Immunofluorescence showed that the expression level of IL-17RA in cell membrane was the highest in the AS model group, and the expression of IL-17RA was decreased in GW4869 group and CD5L group. Expression of inflammatory factors in AS was much higher than that in GW4869 group and CD5L group. The exosomal protein CD5L secreted by macrophage promotes the development of atherosclerosis based on lipid metabolism-induced inflammatory damage of vascular smooth muscle cells.

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来源期刊
CiteScore
5.90
自引率
0.00%
发文量
26
审稿时长
>12 weeks
期刊介绍: Archivum Immunologiae et Therapiae Experimentalis (AITE), founded in 1953 by Ludwik Hirszfeld, is a bimonthly, multidisciplinary journal. It publishes reviews and full original papers dealing with immunology, experimental therapy, immunogenetics, transplantation, microbiology, immunochemistry and ethics in science.
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