{"title":"冈比亚秃鹫中blaCTX-M-15型超广谱β-内酰胺酶的高流行率：一种具有大量人类相互作用的濒危物种","authors":"Hanna Woksepp, Fagimba Camara, Jonas Bonnedahl","doi":"10.1002/mbo3.1349","DOIUrl":null,"url":null,"abstract":"<p>One hundred fecal samples from hooded vultures in the Gambia (Banjul area) were investigated for the presence of bacteria with extended-spectrum cephalosporin- (ESBL/AmpC), carbapenemases, and colistin resistance. No Enterobacteriales carrying carbapenemases or resistance against colistin were detected. Fifty-four ESBL-producing <i>Escherichia coli</i> and five ESBL-producing <i>Klebsiella pneumoniae</i> isolates were identified in 52 of the samples, of which 52 <i>E. coli</i> and 4 <i>K. pneumoniae</i> yielded passed sequencing results. Fifty of the <i>E. coli</i> had ESBL phenotype and genotype harboring <i>bla</i><sub>CTX-M</sub> genes, of which 88.5% (<i>n</i> = 46) were the <i>bla</i><sub>CTX-M-15</sub> gene, commonly found on the African continent. Furthermore, the genetic context around <i>bla</i><sub>CTX-M-15</sub> was similar between isolates, being colocalized with IS<i>Kpn19</i>. In contrast, cgMLST analysis of the <i>E. coli</i> harboring ESBL genes revealed a genetic distribution over a large fraction of the currently known existing <i>E. coli</i> populations in the Gambia. Hooded vultures in the Gambia thus have a high ESBL <i>E. coli</i>-prevalence (>50%) with low diversity regarding key resistance genes. Furthermore, given the urban presence and frequent interactions between hooded vultures and humans, data from this study implies hooded vultures as potential vectors contributing to the further dissemination of antibiotic-resistance genes.</p>","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"12 2","pages":""},"PeriodicalIF":3.9000,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1349","citationCount":"2","resultStr":"{\"title\":\"High prevalence of blaCTX-M-15 type extended-spectrum beta-lactamases in Gambian hooded vultures (Necrosyrtes monachus): A threatened species with substantial human interaction\",\"authors\":\"Hanna Woksepp, Fagimba Camara, Jonas Bonnedahl\",\"doi\":\"10.1002/mbo3.1349\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>One hundred fecal samples from hooded vultures in the Gambia (Banjul area) were investigated for the presence of bacteria with extended-spectrum cephalosporin- (ESBL/AmpC), carbapenemases, and colistin resistance. No Enterobacteriales carrying carbapenemases or resistance against colistin were detected. Fifty-four ESBL-producing <i>Escherichia coli</i> and five ESBL-producing <i>Klebsiella pneumoniae</i> isolates were identified in 52 of the samples, of which 52 <i>E. coli</i> and 4 <i>K. pneumoniae</i> yielded passed sequencing results. Fifty of the <i>E. coli</i> had ESBL phenotype and genotype harboring <i>bla</i><sub>CTX-M</sub> genes, of which 88.5% (<i>n</i> = 46) were the <i>bla</i><sub>CTX-M-15</sub> gene, commonly found on the African continent. Furthermore, the genetic context around <i>bla</i><sub>CTX-M-15</sub> was similar between isolates, being colocalized with IS<i>Kpn19</i>. In contrast, cgMLST analysis of the <i>E. coli</i> harboring ESBL genes revealed a genetic distribution over a large fraction of the currently known existing <i>E. coli</i> populations in the Gambia. Hooded vultures in the Gambia thus have a high ESBL <i>E. coli</i>-prevalence (>50%) with low diversity regarding key resistance genes. Furthermore, given the urban presence and frequent interactions between hooded vultures and humans, data from this study implies hooded vultures as potential vectors contributing to the further dissemination of antibiotic-resistance genes.</p>\",\"PeriodicalId\":18573,\"journal\":{\"name\":\"MicrobiologyOpen\",\"volume\":\"12 2\",\"pages\":\"\"},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2023-03-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1349\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"MicrobiologyOpen\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/mbo3.1349\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"MicrobiologyOpen","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/mbo3.1349","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
High prevalence of blaCTX-M-15 type extended-spectrum beta-lactamases in Gambian hooded vultures (Necrosyrtes monachus): A threatened species with substantial human interaction
One hundred fecal samples from hooded vultures in the Gambia (Banjul area) were investigated for the presence of bacteria with extended-spectrum cephalosporin- (ESBL/AmpC), carbapenemases, and colistin resistance. No Enterobacteriales carrying carbapenemases or resistance against colistin were detected. Fifty-four ESBL-producing Escherichia coli and five ESBL-producing Klebsiella pneumoniae isolates were identified in 52 of the samples, of which 52 E. coli and 4 K. pneumoniae yielded passed sequencing results. Fifty of the E. coli had ESBL phenotype and genotype harboring blaCTX-M genes, of which 88.5% (n = 46) were the blaCTX-M-15 gene, commonly found on the African continent. Furthermore, the genetic context around blaCTX-M-15 was similar between isolates, being colocalized with ISKpn19. In contrast, cgMLST analysis of the E. coli harboring ESBL genes revealed a genetic distribution over a large fraction of the currently known existing E. coli populations in the Gambia. Hooded vultures in the Gambia thus have a high ESBL E. coli-prevalence (>50%) with low diversity regarding key resistance genes. Furthermore, given the urban presence and frequent interactions between hooded vultures and humans, data from this study implies hooded vultures as potential vectors contributing to the further dissemination of antibiotic-resistance genes.
期刊介绍：
MicrobiologyOpen is a peer reviewed, fully open access, broad-scope, and interdisciplinary journal delivering rapid decisions and fast publication of microbial science, a field which is undergoing a profound and exciting evolution in this post-genomic era.
The journal aims to serve the research community by providing a vehicle for authors wishing to publish quality research in both fundamental and applied microbiology. Our goal is to publish articles that stimulate discussion and debate, as well as add to our knowledge base and further the understanding of microbial interactions and microbial processes.
MicrobiologyOpen gives prompt and equal consideration to articles reporting theoretical, experimental, applied, and descriptive work in all aspects of bacteriology, virology, mycology and protistology, including, but not limited to:
- agriculture
- antimicrobial resistance
- astrobiology
- biochemistry
- biotechnology
- cell and molecular biology
- clinical microbiology
- computational, systems, and synthetic microbiology
- environmental science
- evolutionary biology, ecology, and systematics
- food science and technology
- genetics and genomics
- geobiology and earth science
- host-microbe interactions
- infectious diseases
- natural products discovery
- pharmaceutical and medicinal chemistry
- physiology
- plant pathology
- veterinary microbiology
We will consider submissions across unicellular and cell-cluster organisms: prokaryotes (bacteria, archaea) and eukaryotes (fungi, protists, microalgae, lichens), as well as viruses and prions infecting or interacting with microorganisms, plants and animals, including genetic, biochemical, biophysical, bioinformatic and structural analyses.
The journal features Original Articles (including full Research articles, Method articles, and Short Communications), Commentaries, Reviews, and Editorials. Original papers must report well-conducted research with conclusions supported by the data presented in the article. We also support confirmatory research and aim to work with authors to meet reviewer expectations.
MicrobiologyOpen publishes articles submitted directly to the journal and those referred from other Wiley journals.
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