血管生成素-2在脂多糖诱导的幼年SD大鼠急性肺损伤肺组织中的表达及乌司他丁的作用。

IF 2.9 4区 医学 Q3 IMMUNOLOGY Archivum Immunologiae et Therapiae Experimentalis Pub Date : 2023-10-26 DOI:10.1007/s00005-023-00688-7
Junying Qiao, Shanshan Guo, Xianjie Huang, Luodan Zhang, Fan Li, Yazhen Fan
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引用次数: 0

摘要

本研究旨在观察血管生成素-2(Ang-2)在脂多糖(LPS)诱导的急性肺损伤(ALI)幼年SD大鼠肺组织中的表达,并阐明乌司他丁(UTI)的作用。将90只18-21日龄SD雄性幼鼠随机分为5组(n = 18) 。腹腔注射LPS(LPS 10mg/kg)建立ALI大鼠模型,对照组给予相同剂量的生理盐水。UTI干预组在注射LPS后立即注射UTI(5000U/mL),分为UTI低剂量组(LPS + 5 ml/kg UTI)、UTI中剂量组(LPS + 10ml/kg UTI)和UTI高剂量组(LPS + 20ml/kg UTI)。观察各组大鼠的呼吸状态,并随机选择6只大鼠在6、12和24小时处死,解剖并保留肺组织。苏木精-伊红(HE)染色观察肺组织的病理变化、免疫组织化学染色观察Ang-2和血管内皮生长因子(VEGF)在肺组织中的表达水平和位置,采用定量逆转录聚合酶链反应(RT-PCR)和蛋白质印迹法检测Ang-2和VEGF基因和蛋白的表达。腹膜内注射三小时后,模型组大鼠出现呼吸急促,并且随着时间的推移,出现呼吸窘迫。模型组的肺部病理变化与对照组相比明显,并随着时间的推移逐渐恶化,UTI干预组大鼠的肺部病理改变与模型组相比有所减少。在不同时间点,模型组大鼠肺组织中Ang-2和VEGF的表达高于对照组,UTI干预组低于模型组。不同时间点UTI低剂量组Ang-2和VEGF蛋白表达均低于UTI高剂量组(P
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Expression of Angiopoietin-2 in Lung Tissue of Juvenile SD Rats with Lipopolysaccharide-Induced Acute Lung Injury and the Role of Ulinastatin

This study aimed to observe the expression of angiopoietin-2 (Ang-2) in the lung tissue of juvenile SD rats with lipopolysaccharide (LPS)-induced acute lung injury (ALI) and to clarify the role of ulinastatin (UTI). Ninety 18–21-day-old juvenile SD male rats were randomly divided into five groups (n = 18). ALI rat model was established by intraperitoneal injection of LPS (LPS 10 mg/kg), while the control group was given the same dose of normal saline. The UTI intervention group was given the injection of UTI (5000 U/mL) immediately after the injection of LPS, which was divided into UTI low-dose group (LPS + 5 ml/kg UTI), UTI medium-dose group (LPS + 10 ml/kg UTI), and UTI high-dose group (LPS + 20 ml/kg UTI).The respiratory status of each group of rats was observed, and six rats were randomly selected to be killed in each group at 6, 12, and 24 h, and the lung tissues were dissected and retained. The pathological changes of the lung tissues were observed by hematoxylin–eosin (HE) staining, the expression levels and locations of Ang-2 and vascular endothelial growth factor (VEGF) in lung tissue were observed by immunohistochemical staining, and the expressions of genes and proteins of Ang-2 and VEGF were detected by quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot analysis. Three hours after intraperitoneal injection, rats in the model group developed shortness of breath and the developed respiratory distress progressed over time. The lung pathological changes in the model group were obvious compared with those in the control group, and gradually worsened with time, and the pathological changes of lung in the rats in the UTI intervention group were reduced compared with those in the model group. At different time points, the expressions of Ang-2 and VEGF in the lung tissue of rats in the model group were higher than those in the control group, and were lower in the UTI intervention group than those in the model group. The expressions of Ang-2 and VEGF protein were lower in the low-dose group of UTI group than those in the high-dose group of UTI group at different time points (P < 0.05), and the expressions of Ang-2 and VEGF protein in the low-dose group of UTI were significantly lower than those in the medium-dose group at 12 h and 24 h (P < 0.05). The expression of Ang-2 was increased in the lung tissue of juvenile SD rats with LPS-induced ALI, and was associated with the degree of lung injury. UTI might attenuate LPS-induced ALI by inhibiting the expression of Ang-2 in lung tissue, and the low dose was more obvious than the medium and high dose.

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来源期刊
CiteScore
5.90
自引率
0.00%
发文量
26
审稿时长
>12 weeks
期刊介绍: Archivum Immunologiae et Therapiae Experimentalis (AITE), founded in 1953 by Ludwik Hirszfeld, is a bimonthly, multidisciplinary journal. It publishes reviews and full original papers dealing with immunology, experimental therapy, immunogenetics, transplantation, microbiology, immunochemistry and ethics in science.
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