Kwaku Amoah , Beiping Tan , Shuang Zhang , Shuyan Chi , Qihui Yang , Hongyu Liu , Yuanzhi Yang , Haitao Zhang , Xiaohui Dong
{"title":"宿主肠道来源的芽孢杆菌益生菌补充剂可改善杂交石斑鱼的生长性能、血清和肝脏免疫、肠道健康以及抵抗哈维氏弧菌感染的能力(♀富氏石斑鱼×♂柳叶石斑鱼","authors":"Kwaku Amoah , Beiping Tan , Shuang Zhang , Shuyan Chi , Qihui Yang , Hongyu Liu , Yuanzhi Yang , Haitao Zhang , Xiaohui Dong","doi":"10.1016/j.aninu.2023.05.005","DOIUrl":null,"url":null,"abstract":"<div><p>Several reports have revealed the vital role that probiotics play in fish growth and health. However, few works are available for host gut-derived probiotics on the growth, immunity, and gut microbiota of fish, especially in hybrid grouper (♀<em>Epinephelus fuscoguttatus</em> × ♂<em>Epinephelus lanceolatus</em>) due to their isolation difficulty and functional verification. This study aimed at assessing 3 host gut-derived <em>Bacillus</em> species' effects on the growth, immune and antioxidant-biochemical responses, haematological parameters, intestinal morphology, immune-related gene expression, gut microbiota, and disease resistance against <em>Vibrio harveyi</em> in hybrid grouper. A total of 480 hybrid grouper (initial weight = 9.03 ± 0.02 g) were randomly allotted into 4 groups, namely, the group fed a basal diet without probiotic inclusion (control, B0), the group fed the basal diet with <em>Bacillus velezensis</em> GPSAK4 (BV), the group fed the basal diet with <em>Bacillus subtilis</em> GPSAK9 (BS), and the group fed the basal diet with <em>Bacillus tequilensis</em> GPSAK2 (BT) strains at 1.0 × 10<sup>9</sup> CFU/g. After a 6-week feeding trial, the results revealed significant improvements (<em>P</em> < 0.05) in the growth performance, whole fish-body proximate composition, blood haematological parameters, serum, liver, and intestinal biochemical indexes, intestinal morphology, and protection against <em>V</em>. <em>harveyi</em> pathogen in the probiotic-treated groups compared with the untreated. Additionally, the expressions of intestinal tight junction genes (occludin and <em>ZO1</em>), pro- and anti-inflammatory genes, including <em>IL1β</em>, <em>IL6</em>, <em>IL8</em>, <em>TNFα</em>, <em>MyD88</em>, <em>IL10</em>, and <em>TGFβ</em>, were upregulated (<em>P</em> < 0.05) after <em>Bacillus</em> species administration. Host gut-derived <em>Bacillus</em> supplementation shaped the gut microbiota by significantly increasing (<em>P</em> < 0.05) the relative abundance of Proteobacteria, Bacteroidetes, Actinobacteria (except the BS group), Acidobacteria (except the BT group), Cyanobacteria (except the BV and BT groups), and Verrucomicrobia phyla, as well as known beneficial genera (<em>Romboutsia</em>, <em>Turicibacter</em>, <em>Epulopiscium</em>, <em>Clostridium_sensu_stricto</em> 1 and 13, <em>Lactobacillus</em>, and <em>Bacillus</em>), but significantly decreased (<em>P</em> < 0.05) the abundance of Firmicutes, Chloroflexi, and Fusobacteria phyla, and purported pathogenic genera (<em>Staphylococcus</em> and <em>Photobacterium</em>) compared with the control group. Collectively, the results suggest that <em>B</em>. <em>velezensis</em> GPSAK4, <em>B</em>. <em>subtilis</em> GPSAK9 (especially this strain), <em>B</em>. <em>tequilensis</em> GPSAK2 dietary supplementation at 1.0 × 10<sup>9</sup> CFU/g has positive effects on the intestinal health of hybrid grouper via microbial composition modulation, thus enhancing the assimilation and absorption of nutrients to boost fish growth, immunity, and disease resistance.</p></div>","PeriodicalId":62604,"journal":{"name":"Animal Nutrition","volume":null,"pages":null},"PeriodicalIF":6.3000,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Host gut-derived Bacillus probiotics supplementation improves growth performance, serum and liver immunity, gut health, and resistive capacity against Vibrio harveyi infection in hybrid grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus)\",\"authors\":\"Kwaku Amoah , Beiping Tan , Shuang Zhang , Shuyan Chi , Qihui Yang , Hongyu Liu , Yuanzhi Yang , Haitao Zhang , Xiaohui Dong\",\"doi\":\"10.1016/j.aninu.2023.05.005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Several reports have revealed the vital role that probiotics play in fish growth and health. However, few works are available for host gut-derived probiotics on the growth, immunity, and gut microbiota of fish, especially in hybrid grouper (♀<em>Epinephelus fuscoguttatus</em> × ♂<em>Epinephelus lanceolatus</em>) due to their isolation difficulty and functional verification. This study aimed at assessing 3 host gut-derived <em>Bacillus</em> species' effects on the growth, immune and antioxidant-biochemical responses, haematological parameters, intestinal morphology, immune-related gene expression, gut microbiota, and disease resistance against <em>Vibrio harveyi</em> in hybrid grouper. A total of 480 hybrid grouper (initial weight = 9.03 ± 0.02 g) were randomly allotted into 4 groups, namely, the group fed a basal diet without probiotic inclusion (control, B0), the group fed the basal diet with <em>Bacillus velezensis</em> GPSAK4 (BV), the group fed the basal diet with <em>Bacillus subtilis</em> GPSAK9 (BS), and the group fed the basal diet with <em>Bacillus tequilensis</em> GPSAK2 (BT) strains at 1.0 × 10<sup>9</sup> CFU/g. After a 6-week feeding trial, the results revealed significant improvements (<em>P</em> < 0.05) in the growth performance, whole fish-body proximate composition, blood haematological parameters, serum, liver, and intestinal biochemical indexes, intestinal morphology, and protection against <em>V</em>. <em>harveyi</em> pathogen in the probiotic-treated groups compared with the untreated. Additionally, the expressions of intestinal tight junction genes (occludin and <em>ZO1</em>), pro- and anti-inflammatory genes, including <em>IL1β</em>, <em>IL6</em>, <em>IL8</em>, <em>TNFα</em>, <em>MyD88</em>, <em>IL10</em>, and <em>TGFβ</em>, were upregulated (<em>P</em> < 0.05) after <em>Bacillus</em> species administration. Host gut-derived <em>Bacillus</em> supplementation shaped the gut microbiota by significantly increasing (<em>P</em> < 0.05) the relative abundance of Proteobacteria, Bacteroidetes, Actinobacteria (except the BS group), Acidobacteria (except the BT group), Cyanobacteria (except the BV and BT groups), and Verrucomicrobia phyla, as well as known beneficial genera (<em>Romboutsia</em>, <em>Turicibacter</em>, <em>Epulopiscium</em>, <em>Clostridium_sensu_stricto</em> 1 and 13, <em>Lactobacillus</em>, and <em>Bacillus</em>), but significantly decreased (<em>P</em> < 0.05) the abundance of Firmicutes, Chloroflexi, and Fusobacteria phyla, and purported pathogenic genera (<em>Staphylococcus</em> and <em>Photobacterium</em>) compared with the control group. Collectively, the results suggest that <em>B</em>. <em>velezensis</em> GPSAK4, <em>B</em>. <em>subtilis</em> GPSAK9 (especially this strain), <em>B</em>. <em>tequilensis</em> GPSAK2 dietary supplementation at 1.0 × 10<sup>9</sup> CFU/g has positive effects on the intestinal health of hybrid grouper via microbial composition modulation, thus enhancing the assimilation and absorption of nutrients to boost fish growth, immunity, and disease resistance.</p></div>\",\"PeriodicalId\":62604,\"journal\":{\"name\":\"Animal Nutrition\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":6.3000,\"publicationDate\":\"2023-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Animal Nutrition\",\"FirstCategoryId\":\"1091\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2405654523000537\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal Nutrition","FirstCategoryId":"1091","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405654523000537","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Host gut-derived Bacillus probiotics supplementation improves growth performance, serum and liver immunity, gut health, and resistive capacity against Vibrio harveyi infection in hybrid grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus)
Several reports have revealed the vital role that probiotics play in fish growth and health. However, few works are available for host gut-derived probiotics on the growth, immunity, and gut microbiota of fish, especially in hybrid grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus) due to their isolation difficulty and functional verification. This study aimed at assessing 3 host gut-derived Bacillus species' effects on the growth, immune and antioxidant-biochemical responses, haematological parameters, intestinal morphology, immune-related gene expression, gut microbiota, and disease resistance against Vibrio harveyi in hybrid grouper. A total of 480 hybrid grouper (initial weight = 9.03 ± 0.02 g) were randomly allotted into 4 groups, namely, the group fed a basal diet without probiotic inclusion (control, B0), the group fed the basal diet with Bacillus velezensis GPSAK4 (BV), the group fed the basal diet with Bacillus subtilis GPSAK9 (BS), and the group fed the basal diet with Bacillus tequilensis GPSAK2 (BT) strains at 1.0 × 109 CFU/g. After a 6-week feeding trial, the results revealed significant improvements (P < 0.05) in the growth performance, whole fish-body proximate composition, blood haematological parameters, serum, liver, and intestinal biochemical indexes, intestinal morphology, and protection against V. harveyi pathogen in the probiotic-treated groups compared with the untreated. Additionally, the expressions of intestinal tight junction genes (occludin and ZO1), pro- and anti-inflammatory genes, including IL1β, IL6, IL8, TNFα, MyD88, IL10, and TGFβ, were upregulated (P < 0.05) after Bacillus species administration. Host gut-derived Bacillus supplementation shaped the gut microbiota by significantly increasing (P < 0.05) the relative abundance of Proteobacteria, Bacteroidetes, Actinobacteria (except the BS group), Acidobacteria (except the BT group), Cyanobacteria (except the BV and BT groups), and Verrucomicrobia phyla, as well as known beneficial genera (Romboutsia, Turicibacter, Epulopiscium, Clostridium_sensu_stricto 1 and 13, Lactobacillus, and Bacillus), but significantly decreased (P < 0.05) the abundance of Firmicutes, Chloroflexi, and Fusobacteria phyla, and purported pathogenic genera (Staphylococcus and Photobacterium) compared with the control group. Collectively, the results suggest that B. velezensis GPSAK4, B. subtilis GPSAK9 (especially this strain), B. tequilensis GPSAK2 dietary supplementation at 1.0 × 109 CFU/g has positive effects on the intestinal health of hybrid grouper via microbial composition modulation, thus enhancing the assimilation and absorption of nutrients to boost fish growth, immunity, and disease resistance.
期刊介绍:
Animal Nutrition encompasses the full gamut of animal nutritional sciences and reviews including, but not limited to, fundamental aspects of animal nutrition such as nutritional requirements, metabolic studies, body composition, energetics, immunology, neuroscience, microbiology, genetics and molecular and cell biology related to primarily to the nutrition of farm animals and aquatic species. More applied aspects of animal nutrition, such as the evaluation of novel ingredients, feed additives and feed safety will also be considered but it is expected that such studies will have a strong nutritional focus. Animal Nutrition is indexed in SCIE, PubMed Central, Scopus, DOAJ, etc.