宿主肠道来源的芽孢杆菌益生菌补充剂可改善杂交石斑鱼的生长性能、血清和肝脏免疫、肠道健康以及抵抗哈维氏弧菌感染的能力(♀富氏石斑鱼×♂柳叶石斑鱼

IF 6.3 Animal Nutrition Pub Date : 2023-09-01 DOI:10.1016/j.aninu.2023.05.005
Kwaku Amoah , Beiping Tan , Shuang Zhang , Shuyan Chi , Qihui Yang , Hongyu Liu , Yuanzhi Yang , Haitao Zhang , Xiaohui Dong
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引用次数: 0

摘要

一些报告揭示了益生菌在鱼类生长和健康中的重要作用。然而,宿主肠道来源的益生菌对鱼类的生长、免疫和肠道微生物群的研究很少,尤其是在杂交石斑鱼中(♀富氏石斑鱼×♂由于其分离困难和功能验证。本研究旨在评估3种宿主肠道来源的芽孢杆菌对杂交石斑鱼生长、免疫和抗氧化生化反应、血液学参数、肠道形态、免疫相关基因表达、肠道微生物群和对哈维氏弧菌的抗病性的影响。将480条杂交石斑鱼(初始体重=9.03±0.02g)随机分为4组,即:基础日粮不含益生菌的组(对照组,B0)、基础日粮中添加威氏芽孢杆菌GPSAK4(BV)的组、,该组在基础日粮中以1.0×109CFU/g的浓度饲喂龙舌兰杆菌GPSAK2(BT)菌株。经过6周的饲养试验,结果显示,生长性能、全身接近成分、血液学参数、血清、肝脏和肠道生化指标、肠道形态、,以及与未处理组相比,益生菌处理组对哈维氏弧菌病原体的保护作用。此外,肠道紧密连接基因(occludin和ZO1)、促炎和抗炎基因,包括IL1β、IL6、IL8、TNFα、MyD88、IL10和TGFβ的表达在给予芽孢杆菌后上调(P<;0.05)。宿主肠道来源的芽孢杆菌补充通过显著增加(P<;0.05)变形菌门、拟杆菌门、放线菌门(BS组除外)、不动杆菌门(BT组除外)和蓝细菌门(BV和BT组除外,以及已知的有益属(Romboutsia、Turicibacter、Epulopiscium、Clostridium_sensustricto 1和13、乳酸杆菌和芽孢杆菌),但与对照组相比,厚壁菌门、绿弯菌门和梭杆菌门以及所谓的致病属(葡萄球菌和光细菌)的丰度显著降低(P<;0.05)。总之,研究结果表明,1.0×109CFU/g的B.velezensis GPSAK4、B.subtilis GPSAK9(尤其是该菌株)和B.tequilensis GPSAK2日粮补充剂通过调节微生物组成对杂交石斑鱼的肠道健康有积极影响,从而增强营养物质的同化和吸收,促进鱼类的生长、免疫和抗病能力。
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Host gut-derived Bacillus probiotics supplementation improves growth performance, serum and liver immunity, gut health, and resistive capacity against Vibrio harveyi infection in hybrid grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus)

Several reports have revealed the vital role that probiotics play in fish growth and health. However, few works are available for host gut-derived probiotics on the growth, immunity, and gut microbiota of fish, especially in hybrid grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus) due to their isolation difficulty and functional verification. This study aimed at assessing 3 host gut-derived Bacillus species' effects on the growth, immune and antioxidant-biochemical responses, haematological parameters, intestinal morphology, immune-related gene expression, gut microbiota, and disease resistance against Vibrio harveyi in hybrid grouper. A total of 480 hybrid grouper (initial weight = 9.03 ± 0.02 g) were randomly allotted into 4 groups, namely, the group fed a basal diet without probiotic inclusion (control, B0), the group fed the basal diet with Bacillus velezensis GPSAK4 (BV), the group fed the basal diet with Bacillus subtilis GPSAK9 (BS), and the group fed the basal diet with Bacillus tequilensis GPSAK2 (BT) strains at 1.0 × 109 CFU/g. After a 6-week feeding trial, the results revealed significant improvements (P < 0.05) in the growth performance, whole fish-body proximate composition, blood haematological parameters, serum, liver, and intestinal biochemical indexes, intestinal morphology, and protection against V. harveyi pathogen in the probiotic-treated groups compared with the untreated. Additionally, the expressions of intestinal tight junction genes (occludin and ZO1), pro- and anti-inflammatory genes, including IL1β, IL6, IL8, TNFα, MyD88, IL10, and TGFβ, were upregulated (P < 0.05) after Bacillus species administration. Host gut-derived Bacillus supplementation shaped the gut microbiota by significantly increasing (P < 0.05) the relative abundance of Proteobacteria, Bacteroidetes, Actinobacteria (except the BS group), Acidobacteria (except the BT group), Cyanobacteria (except the BV and BT groups), and Verrucomicrobia phyla, as well as known beneficial genera (Romboutsia, Turicibacter, Epulopiscium, Clostridium_sensu_stricto 1 and 13, Lactobacillus, and Bacillus), but significantly decreased (P < 0.05) the abundance of Firmicutes, Chloroflexi, and Fusobacteria phyla, and purported pathogenic genera (Staphylococcus and Photobacterium) compared with the control group. Collectively, the results suggest that B. velezensis GPSAK4, B. subtilis GPSAK9 (especially this strain), B. tequilensis GPSAK2 dietary supplementation at 1.0 × 109 CFU/g has positive effects on the intestinal health of hybrid grouper via microbial composition modulation, thus enhancing the assimilation and absorption of nutrients to boost fish growth, immunity, and disease resistance.

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来源期刊
Animal Nutrition
Animal Nutrition Animal Science and Zoology
CiteScore
9.70
自引率
0.00%
发文量
542
审稿时长
65 days
期刊介绍: Animal Nutrition encompasses the full gamut of animal nutritional sciences and reviews including, but not limited to, fundamental aspects of animal nutrition such as nutritional requirements, metabolic studies, body composition, energetics, immunology, neuroscience, microbiology, genetics and molecular and cell biology related to primarily to the nutrition of farm animals and aquatic species. More applied aspects of animal nutrition, such as the evaluation of novel ingredients, feed additives and feed safety will also be considered but it is expected that such studies will have a strong nutritional focus. Animal Nutrition is indexed in SCIE, PubMed Central, Scopus, DOAJ, etc.
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