在白色念珠菌和其他酵母菌中,营养消耗和TOR抑制诱导18S和25S核糖体rna抵抗5 ' -磷酸依赖性核酸外切酶

IF 2.946 Q3 Biochemistry, Genetics and Molecular Biology BMC Molecular Biology Pub Date : 2018-01-19 DOI:10.1186/s12867-018-0102-y
Jacob Fleischmann, Miguel A. Rocha
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引用次数: 7

摘要

信使RNA (mRNA)代表了细胞中一小部分RNA,核糖体RNA (rRNA)构成了大部分RNA。为了从真核生物中分离mRNA,通常进行poly-A选择。最近,一种5 ' -磷酸依赖的5 '→3 '过程外切酶被称为终结者。它只会消化具有5 ' -单磷酸末端的RNA,因此它对消除细胞中的大多数rrna非常有用。我们发现,在白色念珠菌中,从健壮生长期的酵母中分离出的18S和25S组分很容易被终结者酶消除,而从营养减少的静止期细胞中分离出的18S和25S组分对该酶产生抗性。碱性磷酸酶、烟草焦磷酸酶联合终结者酶的额外消化指向18S和25S的5 ' - '端作为这种抗性的来源。雷帕霉素对TOR的抑制也会诱导这些分子产生耐药性。我们还发现这些分子与核糖体结合,并不是偶然产生的。最后,我们证明了另外三种酵母也表现出同样的行为。用终结者酶切RNA,发现18S和25S rRNA分子不同于核糖体生成中被接受的加工过的RNA分子。这些分子产生的原因及其形成的潜在机制尚不清楚。在这些酵母中保存这种行为表明它具有有用的生物学作用,值得进一步研究。
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Nutrient depletion and TOR inhibition induce 18S and 25S ribosomal RNAs resistant to a 5′-phosphate-dependent exonuclease in Candida albicans and other yeasts

Messenger RNA (mRNA) represents a small percentage of RNAs in a cell, with ribosomal RNA (rRNA) making up the bulk of it. To isolate mRNA from eukaryotes, typically poly-A selection is carried out. Recently, a 5′-phosphate-dependent, 5′→3′ processive exonuclease called Terminator has become available. It will digest only RNA that has a 5′-monophosphate end and therefore it is very useful to eliminate most of rRNAs in cell.

We have found that in the pathogenic yeast Candida albicans, while 18S and 25S components isolated from yeast in robust growth phase are easily eliminated by Terminator, those isolated from cells in the nutritionally diminished stationary phase, become resistant to digestion by this enzyme. Additional digestions with alkaline phosphatase, tobacco pyrophosphatase combined with Terminator point toward the 5′-prime end of 18S and 25S as the source of this resistance. Inhibition of TOR by rapamycin also induces resistance by these molecules. We also find that these molecules are incorporated into the ribosome and are not just produced incidentally. Finally, we show that three other yeasts show the same behavior.

Digestion of RNA by Terminator has revealed 18S and 25S rRNA molecules different from the accepted processed ones seen in ribosome generation. The reason for these molecules and the underlying mechanism for their formation is unknown. The preservation of this behavior across these yeasts suggests a useful biological role for it, worthy of further inquiry.

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来源期刊
BMC Molecular Biology
BMC Molecular Biology 生物-生化与分子生物学
CiteScore
4.80
自引率
0.00%
发文量
0
审稿时长
>12 weeks
期刊介绍: BMC Molecular Biology is an open access journal publishing original peer-reviewed research articles in all aspects of DNA and RNA in a cellular context, encompassing investigations of chromatin, replication, recombination, mutation, repair, transcription, translation and RNA processing and function.
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