Overview of qPCR Molecular Probes

In recent times, varieties of modified synthetic oligonucleotides conjugated with reporter molecules have been employed in hybridization-based assays. These methods have broad applications in both basic molecular biology research and in clinical diagnostics and screening because they have high specificity to a target nucleic acid sequence. The development of the fluorescence resonance energy transfer (FRET) process has improved the performance and utility of these hybridization-based assays. In this unit, we discuss the main principles of the design and synthesis of the qPCR probes. © 2015 by John Wiley & Sons, Inc.