Brian Flatley , Chris Quaye , Elizabeth Johnson , Alex Freeman , Asif Muneer , Suks Minhas , Jennifer C. Paterson , Fawaz Musa , Peter Malone , Rainer Cramer
{"title":"假定癌症标志物S100A4在浸润性鳞状细胞癌阴茎组织中的分布分析","authors":"Brian Flatley , Chris Quaye , Elizabeth Johnson , Alex Freeman , Asif Muneer , Suks Minhas , Jennifer C. Paterson , Fawaz Musa , Peter Malone , Rainer Cramer","doi":"10.1016/j.euprot.2015.02.001","DOIUrl":null,"url":null,"abstract":"<div><p>MS-based proteomic methods were utilised for the first time in the discovery of novel penile cancer biomarkers. MALDI MS imaging was used to obtain the in situ biomolecular MS profile of squamous cell carcinoma of the penis which was then compared to benign epithelial MS profiles. Spectra from cancerous and benign tissue areas were examined to identify MS peaks that best distinguished normal epithelial cells from invasive squamous epithelial cells, providing crucial evidence to suggest S100A4 to be differentially expressed. Verification by immunohistochemistry resulted in positive staining for S100A4 in a sub-population of invasive but not benign epithelial cells.</p></div>","PeriodicalId":38260,"journal":{"name":"EuPA Open Proteomics","volume":"7 ","pages":"Pages 1-10"},"PeriodicalIF":0.0000,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.euprot.2015.02.001","citationCount":"2","resultStr":"{\"title\":\"Distribution analysis of the putative cancer marker S100A4 across invasive squamous cell carcinoma penile tissue\",\"authors\":\"Brian Flatley , Chris Quaye , Elizabeth Johnson , Alex Freeman , Asif Muneer , Suks Minhas , Jennifer C. Paterson , Fawaz Musa , Peter Malone , Rainer Cramer\",\"doi\":\"10.1016/j.euprot.2015.02.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>MS-based proteomic methods were utilised for the first time in the discovery of novel penile cancer biomarkers. MALDI MS imaging was used to obtain the in situ biomolecular MS profile of squamous cell carcinoma of the penis which was then compared to benign epithelial MS profiles. Spectra from cancerous and benign tissue areas were examined to identify MS peaks that best distinguished normal epithelial cells from invasive squamous epithelial cells, providing crucial evidence to suggest S100A4 to be differentially expressed. Verification by immunohistochemistry resulted in positive staining for S100A4 in a sub-population of invasive but not benign epithelial cells.</p></div>\",\"PeriodicalId\":38260,\"journal\":{\"name\":\"EuPA Open Proteomics\",\"volume\":\"7 \",\"pages\":\"Pages 1-10\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2015-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.euprot.2015.02.001\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"EuPA Open Proteomics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2212968515000057\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"EuPA Open Proteomics","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212968515000057","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
Distribution analysis of the putative cancer marker S100A4 across invasive squamous cell carcinoma penile tissue
MS-based proteomic methods were utilised for the first time in the discovery of novel penile cancer biomarkers. MALDI MS imaging was used to obtain the in situ biomolecular MS profile of squamous cell carcinoma of the penis which was then compared to benign epithelial MS profiles. Spectra from cancerous and benign tissue areas were examined to identify MS peaks that best distinguished normal epithelial cells from invasive squamous epithelial cells, providing crucial evidence to suggest S100A4 to be differentially expressed. Verification by immunohistochemistry resulted in positive staining for S100A4 in a sub-population of invasive but not benign epithelial cells.
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