Melinda Rezeli , Henrik Zetterberg , Kaj Blennow , Ann Brinkmalm , Thomas Laurell , Oskar Hansson , György Marko-Varga
{"title":"阿尔茨海默病和其他神经退行性疾病患者脑脊液和血液中总载脂蛋白E及其特异性亚型的定量分析","authors":"Melinda Rezeli , Henrik Zetterberg , Kaj Blennow , Ann Brinkmalm , Thomas Laurell , Oskar Hansson , György Marko-Varga","doi":"10.1016/j.euprot.2015.07.012","DOIUrl":null,"url":null,"abstract":"<div><p>A targeted mass spectrometric assay was developed for identification and quantification of apoE isoforms (apoE2, E3 and E4), and it was utilized for screening of samples from AD patients (<em>n</em> <!-->=<!--> <!-->39) and patients with other neurodegenerative disorders (<em>n</em> <!-->=<!--> <!-->38). The assay showed good linearity with LOQ corresponds to total apoE concentration of 0.8 and 40<!--> <!-->ng/mL in CSF and plasma/serum, respectively. We identified apoE phenotypes with 100% accuracy in clinical samples. We found strong association between genotypes of the individuals and their apoE levels in blood; ϵ4 allele carriers had significantly lower apoE levels in blood than non-carriers.</p></div>","PeriodicalId":38260,"journal":{"name":"EuPA Open Proteomics","volume":"8 ","pages":"Pages 137-143"},"PeriodicalIF":0.0000,"publicationDate":"2015-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.euprot.2015.07.012","citationCount":"36","resultStr":"{\"title\":\"Quantification of total apolipoprotein E and its specific isoforms in cerebrospinal fluid and blood in Alzheimer’s disease and other neurodegenerative diseases\",\"authors\":\"Melinda Rezeli , Henrik Zetterberg , Kaj Blennow , Ann Brinkmalm , Thomas Laurell , Oskar Hansson , György Marko-Varga\",\"doi\":\"10.1016/j.euprot.2015.07.012\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A targeted mass spectrometric assay was developed for identification and quantification of apoE isoforms (apoE2, E3 and E4), and it was utilized for screening of samples from AD patients (<em>n</em> <!-->=<!--> <!-->39) and patients with other neurodegenerative disorders (<em>n</em> <!-->=<!--> <!-->38). The assay showed good linearity with LOQ corresponds to total apoE concentration of 0.8 and 40<!--> <!-->ng/mL in CSF and plasma/serum, respectively. We identified apoE phenotypes with 100% accuracy in clinical samples. We found strong association between genotypes of the individuals and their apoE levels in blood; ϵ4 allele carriers had significantly lower apoE levels in blood than non-carriers.</p></div>\",\"PeriodicalId\":38260,\"journal\":{\"name\":\"EuPA Open Proteomics\",\"volume\":\"8 \",\"pages\":\"Pages 137-143\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2015-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.euprot.2015.07.012\",\"citationCount\":\"36\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"EuPA Open Proteomics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2212968515300143\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"EuPA Open Proteomics","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212968515300143","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
Quantification of total apolipoprotein E and its specific isoforms in cerebrospinal fluid and blood in Alzheimer’s disease and other neurodegenerative diseases
A targeted mass spectrometric assay was developed for identification and quantification of apoE isoforms (apoE2, E3 and E4), and it was utilized for screening of samples from AD patients (n = 39) and patients with other neurodegenerative disorders (n = 38). The assay showed good linearity with LOQ corresponds to total apoE concentration of 0.8 and 40 ng/mL in CSF and plasma/serum, respectively. We identified apoE phenotypes with 100% accuracy in clinical samples. We found strong association between genotypes of the individuals and their apoE levels in blood; ϵ4 allele carriers had significantly lower apoE levels in blood than non-carriers.
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