{"title":"一种与viunalikevvirus密切相关的真菌噬菌体(Ralstonia solanacearum噬菌体)可编码19个trna相关序列","authors":"Tomoko Mihara , Mohamed A. Nasr-Eldin , Orawan Chatchawankanphanich , Anjana Bhunchoth , Namthip Phironrit , Takeru Kawasaki , Miyako Nakano , Makoto Fujie , Hiroyuki Ogata , Takashi Yamada","doi":"10.1016/j.virep.2016.07.001","DOIUrl":null,"url":null,"abstract":"<div><p>A T4-related phage infecting <em>Ralstonia solanacearum</em> was isolated in Thailand (ϕRP15). ϕRP15 virions showed unique morphology with star-like protrusions attached to the base plate via a stalk. The 168<!--> <!-->kb genome sequence of ϕRP15 was determined and found to encode 17 tRNAs (plus two tRNA pseudogenes). Ten of these phage-encoded tRNAs corresponded to codons that are relatively abundant in the phage but rare in the host, while others matched to codons frequent in both phage and host. Phylogenetic and proteomic analyses demonstrate that ϕRP15 forms a clade with <em>Delftia</em> phage ϕW-14, which in turn is placed as a sister group of viunalikeviruses. The hosts of ϕRP15 and ϕW-14 (<em>R. solanacearum</em> and <em>Delftia acidovorans</em>, respectively) belong to Betaproteobacteria, while most of the hosts of viunalikeviruses are of the <em>Enterobacteriaceae</em> belonging to Gammaproteobacteria. These phages may have evolved closely associated with their hosts that have very different life-styles in the natural habitats.</p></div>","PeriodicalId":90762,"journal":{"name":"Virology reports","volume":"6 ","pages":"Pages 61-73"},"PeriodicalIF":0.0000,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.virep.2016.07.001","citationCount":"5","resultStr":"{\"title\":\"A Ralstonia solanacearum phage ϕRP15 is closely related to Viunalikeviruses and encodes 19 tRNA-related sequences\",\"authors\":\"Tomoko Mihara , Mohamed A. Nasr-Eldin , Orawan Chatchawankanphanich , Anjana Bhunchoth , Namthip Phironrit , Takeru Kawasaki , Miyako Nakano , Makoto Fujie , Hiroyuki Ogata , Takashi Yamada\",\"doi\":\"10.1016/j.virep.2016.07.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A T4-related phage infecting <em>Ralstonia solanacearum</em> was isolated in Thailand (ϕRP15). ϕRP15 virions showed unique morphology with star-like protrusions attached to the base plate via a stalk. The 168<!--> <!-->kb genome sequence of ϕRP15 was determined and found to encode 17 tRNAs (plus two tRNA pseudogenes). Ten of these phage-encoded tRNAs corresponded to codons that are relatively abundant in the phage but rare in the host, while others matched to codons frequent in both phage and host. Phylogenetic and proteomic analyses demonstrate that ϕRP15 forms a clade with <em>Delftia</em> phage ϕW-14, which in turn is placed as a sister group of viunalikeviruses. The hosts of ϕRP15 and ϕW-14 (<em>R. solanacearum</em> and <em>Delftia acidovorans</em>, respectively) belong to Betaproteobacteria, while most of the hosts of viunalikeviruses are of the <em>Enterobacteriaceae</em> belonging to Gammaproteobacteria. These phages may have evolved closely associated with their hosts that have very different life-styles in the natural habitats.</p></div>\",\"PeriodicalId\":90762,\"journal\":{\"name\":\"Virology reports\",\"volume\":\"6 \",\"pages\":\"Pages 61-73\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2016-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.virep.2016.07.001\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Virology reports\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2214669516300101\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Virology reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214669516300101","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5
摘要
在泰国分离到一株与结核分枝杆菌相关的噬菌体(ϕRP15)。rp15病毒粒子表现出独特的形态，其星状突起通过茎附着在基板上。测定了其168 kb的基因组序列，发现其编码17个tRNA(外加2个tRNA假基因)。这些噬菌体编码的trna中有10个与噬菌体中相对丰富但在宿主中罕见的密码子相对应，而其他trna则与噬菌体和宿主中都常见的密码子相匹配。系统发育和蛋白质组学分析表明， rp15与Delftia噬菌体 w -14形成一个分支，而后者又被放置为viunalikevvirus的姐妹群。大肠杆菌的寄主(R. solanacearum)和大肠杆菌的寄主(R. solanacearum)和大肠杆菌的寄主(Delftia acidovorans)分别属于Betaproteobacteria，而viunalikevuses的寄主大多属于肠杆菌科的Gammaproteobacteria。这些噬菌体可能与它们的宿主密切相关，而宿主在自然栖息地中有着截然不同的生活方式。
A Ralstonia solanacearum phage ϕRP15 is closely related to Viunalikeviruses and encodes 19 tRNA-related sequences
A T4-related phage infecting Ralstonia solanacearum was isolated in Thailand (ϕRP15). ϕRP15 virions showed unique morphology with star-like protrusions attached to the base plate via a stalk. The 168 kb genome sequence of ϕRP15 was determined and found to encode 17 tRNAs (plus two tRNA pseudogenes). Ten of these phage-encoded tRNAs corresponded to codons that are relatively abundant in the phage but rare in the host, while others matched to codons frequent in both phage and host. Phylogenetic and proteomic analyses demonstrate that ϕRP15 forms a clade with Delftia phage ϕW-14, which in turn is placed as a sister group of viunalikeviruses. The hosts of ϕRP15 and ϕW-14 (R. solanacearum and Delftia acidovorans, respectively) belong to Betaproteobacteria, while most of the hosts of viunalikeviruses are of the Enterobacteriaceae belonging to Gammaproteobacteria. These phages may have evolved closely associated with their hosts that have very different life-styles in the natural habitats.
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