Light microscopic study on the tumor cell-associated antigens in patients with breast cancer by immunoperoxidase technique

Tumors from patients with breast cancer were examined by light microscopy using immunoperoxidase techniques. The positive immunoperoxidase reaction was observed in 12 of 19 tumors treated with autologous serum. The percentage of the labeled tumor cells was from 10–15 to 60–70. It was established that tumor cell-associated antigen(s) was located mainly on both, the plasma and the nuclear membrane. More rarely it could be seen on the chromatin.