A guided experimental approach to practical molecular pharmacology teaching: demonstration of sequence selectivity of DNA-binding drugs by arrested polymerase chain reaction

Here, we report a simple experimental approach for demonstrating sequence-selectivity of DNA-binding drugs by using the polymerase-chain reaction (PCR). The experiments described could be easily and reproducibly performed by medical and science students and biochemistry teachers, do not require complex or expensive instruments and ³²P labelled probes or primers. The procedure described could represent a novel approach to practical biochemistry teaching in the field of biochemical pharma-cology.