Lan QI , Wen-Quan WANG , Zhen-Wen ZHANG , Jian-Qiu YE , Kai-Mian LI
{"title":"基于序列相关扩增多态性标记的18个木薯品种DNA指纹图谱","authors":"Lan QI , Wen-Quan WANG , Zhen-Wen ZHANG , Jian-Qiu YE , Kai-Mian LI","doi":"10.1016/S1875-2780(09)60076-6","DOIUrl":null,"url":null,"abstract":"<div><p>Thirty-six pairs of polymorphic SRAP markers were used to construct DNA fingerprint of 18 cassava (<em>Manihot esculenta</em> Crantz) varieties. Among the total 320 amplified bands, 235 (73.4%) were polymorphic with an average of 6.5 bands per primer pair. The unweighted pair-group method with arithmetic average (UPGMA) cluster showed 4 major groups at similarity index of 0.734. Two primer combinations, Me1/Em5 and Me24/Em10, had the highest efficiency to distinguish cassava varieties, which were applicable to develop the DNA fingerprints of the 18 cassava varieties. The amplified bands were converted into binary codes with presence (1) and absence (0), and each variety had a unique 18-digital code. The confident probability of the DNA fingerprint was as high as 99.9996%.</p></div>","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2010-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1875-2780(09)60076-6","citationCount":"3","resultStr":"{\"title\":\"DNA Fingerprints of 18 Cassava Varieties Based on Sequence-Related Amplified Polymorphism Markers\",\"authors\":\"Lan QI , Wen-Quan WANG , Zhen-Wen ZHANG , Jian-Qiu YE , Kai-Mian LI\",\"doi\":\"10.1016/S1875-2780(09)60076-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Thirty-six pairs of polymorphic SRAP markers were used to construct DNA fingerprint of 18 cassava (<em>Manihot esculenta</em> Crantz) varieties. Among the total 320 amplified bands, 235 (73.4%) were polymorphic with an average of 6.5 bands per primer pair. The unweighted pair-group method with arithmetic average (UPGMA) cluster showed 4 major groups at similarity index of 0.734. Two primer combinations, Me1/Em5 and Me24/Em10, had the highest efficiency to distinguish cassava varieties, which were applicable to develop the DNA fingerprints of the 18 cassava varieties. The amplified bands were converted into binary codes with presence (1) and absence (0), and each variety had a unique 18-digital code. The confident probability of the DNA fingerprint was as high as 99.9996%.</p></div>\",\"PeriodicalId\":7085,\"journal\":{\"name\":\"Acta Agronomica Sinica\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2010-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S1875-2780(09)60076-6\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Agronomica Sinica\",\"FirstCategoryId\":\"1091\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1875278009600766\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Agronomica Sinica","FirstCategoryId":"1091","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1875278009600766","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
DNA Fingerprints of 18 Cassava Varieties Based on Sequence-Related Amplified Polymorphism Markers
Thirty-six pairs of polymorphic SRAP markers were used to construct DNA fingerprint of 18 cassava (Manihot esculenta Crantz) varieties. Among the total 320 amplified bands, 235 (73.4%) were polymorphic with an average of 6.5 bands per primer pair. The unweighted pair-group method with arithmetic average (UPGMA) cluster showed 4 major groups at similarity index of 0.734. Two primer combinations, Me1/Em5 and Me24/Em10, had the highest efficiency to distinguish cassava varieties, which were applicable to develop the DNA fingerprints of the 18 cassava varieties. The amplified bands were converted into binary codes with presence (1) and absence (0), and each variety had a unique 18-digital code. The confident probability of the DNA fingerprint was as high as 99.9996%.
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