水稻OsASIE1基因在非生物胁迫下的功能研究

Q3 Agricultural and Biological Sciences Acta Agronomica Sinica Pub Date : 2011-10-01 DOI:10.1016/S1875-2780(11)60048-5
Hui-Min WU, Li-Yu HUANG, Ya-Jiao PAN, Peng JIN, Bin-Ying FU
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引用次数: 1

摘要

AP2/EREBP转录因子在植物发育、激素响应以及生物和非生物胁迫响应中发挥重要作用。OsASIE1是水稻AP2/EREBP转录因子EREBP亚家族的成员,被确定参与非生物胁迫应答。干旱和盐胁迫诱导OsASIE1表达。此外,电泳迁移迁移分析(EMSA)显示,OsASIE1蛋白的AP2结构域在体外可以结合DRE(脱水响应元件)和GCC box(乙烯响应元件,ERE)。这些结果表明,OsASIE1可能通过DRE和GCC盒子结合调控下游基因的表达参与非生物胁迫应答。OsASIE1基因在转基因水稻中的过表达可能用于提高对盐胁迫的耐受性。
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Function of Gene OsASIE1 in Response to Abiotic Stress in Rice

AP2/EREBP transcription factors play an important role in plant development, hormone response, and responses to biotic and abiotic stress. OsASIE1, a member of the EREBP subfamily of AP2/EREBP transcription factors in rice (Oryza sativa L.), is determined to be involved in abiotic stress response. OsASIE1 expression was induced by drought and salt stresses. In addition, an electrophoresis mobility shift assay (EMSA) revealed that the AP2 domain of the OsASIE1 protein can bind to both DRE (dehydration-responsive element) and GCC box (ethylene response element, ERE) in vitro. All these results indicate that OsASIE1 may participate in abiotic stress response by regulating the expression of downstream genes with DRE and GCC box binding. Overexpression of OsASIE1 in transgenic rice plants might be used to improve tolerance to salt stress.

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CiteScore
1.50
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审稿时长
30 weeks
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