F. Ndoye, M. S. Yousafzai, G. Coceano, S. Bonin, G. Scoles, O. Ka, J. Niemela, D. Cojoc
{"title":"横向力对光镊垂直压痕测量单元刚度的影响","authors":"F. Ndoye, M. S. Yousafzai, G. Coceano, S. Bonin, G. Scoles, O. Ka, J. Niemela, D. Cojoc","doi":"10.1080/15599612.2016.1149896","DOIUrl":null,"url":null,"abstract":"ABSTRACT We studied the lateral forces arising during the vertical indentation of the cell membrane by an optically trapped microbead, using back focal plane interferometry to determine force components in all directions. We analyzed the cell-microbead interaction and showed that indeed the force had also lateral components. Using the Hertz model, we calculated and compared the elastic moduli resulting from the total and vertical forces, showing that the differences are important and the total force should be considered. To confirm our results we analyzed cells from two breast cancer cell lines: MDA-MB-231 and HBL-100, known to have different cancer aggressiveness and hence stiffness.","PeriodicalId":50296,"journal":{"name":"International Journal of Optomechatronics","volume":"10 1","pages":"53 - 62"},"PeriodicalIF":6.7000,"publicationDate":"2016-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15599612.2016.1149896","citationCount":"10","resultStr":"{\"title\":\"The influence of lateral forces on the cell stiffness measurement by optical tweezers vertical indentation\",\"authors\":\"F. Ndoye, M. S. Yousafzai, G. Coceano, S. Bonin, G. Scoles, O. Ka, J. Niemela, D. Cojoc\",\"doi\":\"10.1080/15599612.2016.1149896\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"ABSTRACT We studied the lateral forces arising during the vertical indentation of the cell membrane by an optically trapped microbead, using back focal plane interferometry to determine force components in all directions. We analyzed the cell-microbead interaction and showed that indeed the force had also lateral components. Using the Hertz model, we calculated and compared the elastic moduli resulting from the total and vertical forces, showing that the differences are important and the total force should be considered. To confirm our results we analyzed cells from two breast cancer cell lines: MDA-MB-231 and HBL-100, known to have different cancer aggressiveness and hence stiffness.\",\"PeriodicalId\":50296,\"journal\":{\"name\":\"International Journal of Optomechatronics\",\"volume\":\"10 1\",\"pages\":\"53 - 62\"},\"PeriodicalIF\":6.7000,\"publicationDate\":\"2016-01-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1080/15599612.2016.1149896\",\"citationCount\":\"10\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Optomechatronics\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1080/15599612.2016.1149896\",\"RegionNum\":3,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, ELECTRICAL & ELECTRONIC\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Optomechatronics","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1080/15599612.2016.1149896","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
The influence of lateral forces on the cell stiffness measurement by optical tweezers vertical indentation
ABSTRACT We studied the lateral forces arising during the vertical indentation of the cell membrane by an optically trapped microbead, using back focal plane interferometry to determine force components in all directions. We analyzed the cell-microbead interaction and showed that indeed the force had also lateral components. Using the Hertz model, we calculated and compared the elastic moduli resulting from the total and vertical forces, showing that the differences are important and the total force should be considered. To confirm our results we analyzed cells from two breast cancer cell lines: MDA-MB-231 and HBL-100, known to have different cancer aggressiveness and hence stiffness.
期刊介绍：
International Journal of Optomechatronics publishes the latest results of multidisciplinary research at the crossroads between optics, mechanics, fluidics and electronics.
Topics you can submit include, but are not limited to:
-Adaptive optics-
Optomechanics-
Machine vision, tracking and control-
Image-based micro-/nano- manipulation-
Control engineering for optomechatronics-
Optical metrology-
Optical sensors and light-based actuators-
Optomechatronics for astronomy and space applications-
Optical-based inspection and fault diagnosis-
Micro-/nano- optomechanical systems (MOEMS)-
Optofluidics-
Optical assembly and packaging-
Optical and vision-based manufacturing, processes, monitoring, and control-
Optomechatronics systems in bio- and medical technologies (such as optical coherence tomography (OCT) systems or endoscopes and optical based medical instruments)
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
