一种铜绿假单胞菌酰胺酶特异性单克隆抗体。

Hybridoma Pub Date : 2001-08-01 DOI:10.1089/027245701753179866

C. Novo, A. Karmali, A. Clemente, P. Brown

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引用次数: 5

摘要

采用阴离子交换层析纯化铜绿假单胞菌的氨基酶，并用于雌性Balb/c小鼠免疫。以Sp2/0骨髓瘤细胞为融合体，采用杂交瘤技术制备单克隆抗体(mab)。采用两步阴离子交换色谱法从体外杂交瘤细胞系上清中纯化出一个IgM亚类单抗。该单抗对铜绿假单胞菌的氨基酶具有特异性，可识别该酶的天然和变性形式。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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A monoclonal antibody specific for Pseudomonas aeruginosa amidase.

Amidase from Pseudomonas aeruginosa was purified by anionic exchange chromatography and used to immunise female Balb/c mice. Monoclonal antibodies (MAbs) were raised by hybridoma technology using Sp2/0 myeloma cells as fusion partner. A selected IgM subclass MAb was purified from in vitro hybridoma cell line supernatant by a two-step anionic exchange chromatography. The MAb was specific for amidase from P. aeruginosa as determined by Western blotting and recognized the native and denatured forms of the enzyme.

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