腺相关病毒载体在三维切片培养中转导成熟的人脂肪细胞。

Q1 Immunology and Microbiology Human Gene Therapy Methods Pub Date : 2016-10-01 DOI:10.1089/HGTB.2016.137

KallendruschSonja, SchopowNikolas, C. StadlerSonja, BüningHildegard, T. HackerUlrich

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引用次数: 1

摘要

脂肪组织作为一种内分泌器官，在调节体内能量平衡方面起着举足轻重的作用。因此，脂肪组织功能障碍与胰岛素抵抗、病态肥胖和代谢综合征密切相关。为了研究分子机制和开发新的治疗策略，需要对成熟脂肪细胞进行基因修饰的技术。在这里，我们报道了腺相关病毒(AAV)载体作为一种多功能工具，在器官型三维组织培养中转导人类成熟脂肪细胞。

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Adeno-Associated Viral Vectors Transduce Mature Human Adipocytes in Three-Dimensional Slice Cultures.

Adipose tissue plays a pivotal role, both in the regulation of energy homeostasis and as an endocrine organ. Consequently, adipose tissue dysfunction is closely related to insulin resistance, morbid obesity, and metabolic syndrome. To study molecular mechanisms and to develop novel therapeutic strategies, techniques are required to genetically modify mature adipocytes. Here, we report on adeno-associated viral (AAV) vectors as a versatile tool to transduce human mature adipocytes in organotypic three-dimensional tissue cultures.

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来源期刊

Human Gene Therapy Methods BIOTECHNOLOGY & APPLIED MICROBIOLOGY-GENETICS & HEREDITY

CiteScore

5.80

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Human Gene Therapy is the premier, multidisciplinary journal covering all aspects of gene therapy. The Journal publishes in-depth coverage of DNA, RNA, and cell therapies by delivering the latest breakthroughs in research and technologies. Human Gene Therapy provides a central forum for scientific and clinical information, including ethical, legal, regulatory, social, and commercial issues, which enables the advancement and progress of therapeutic procedures leading to improved patient outcomes, and ultimately, to curing diseases. The Journal is divided into three parts. Human Gene Therapy, the flagship, is published 12 times per year. HGT Methods, a bimonthly journal, focuses on the applications of gene therapy to product testing and development. HGT Clinical Development, a quarterly journal, serves as a venue for publishing data relevant to the regulatory review and commercial development of cell and gene therapy products.