EFFECTS OF DIETARY PLANT CEREBROSIDE ON GENE EXPRESSION IN THE LARGE INTESTINE OF 1,2-DIMETHYLHYDRAZINE (DMH)-TREATED MICE DETERMINED BY DNA MICROARRAY ANALYSIS

ABSTRACT

The effects of dietary plant cerebroside on colon gene expression by DNA microarray analysis in 1,2-dimethylhydrazine (DMH)-treated mice were investigated. After 9 weeks of feeding with DMH, decreases and increases in the levels of expression of 110 and 145 genes were detected, respectively. Especially, the expression of Soggy-1, which suppresses the Wnt signaling pathway, was increased, while that of Ras-associated protein, which induces the mitogen-activated protein (MAP)-kinase pathway and is responsible for the development of aberrant crypt foci (ACF), was decreased. The results of the present study indicated that dietary cerebroside in DMH-treated mice regulates the Wnt signaling and MAP-kinase pathways, and prevents the development of ACF in the large intestine.

PRACTICAL APPLICATIONS

Dietary plant cerebroside increased expression of Soggy-1 mRNA, which suppresses the Wnt signaling pathway, and decreased expression ofRas-associated protein, which induces the MAP-kinase pathway in 1,2-dimethylhydrazine (DMH)-treated mice. The results of the present study indicated that dietary cerebroside in DMH-treated mice regulates the Wnt signaling and MAP-kinase pathways, and prevents the development of aberrant crypt foci in the large intestine, thus being of potential use for nutraceutical applications.