肠道菌群和饲粮植酸对无菌和常规大鼠肠道植酸酶活性的影响

S. Shinoda, Tsutomu Yoshida
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引用次数: 1

摘要

无菌大鼠和常规大鼠分别饲喂基础或2%植酸饲料。肠道菌群对粘膜植酸酶活性无明显影响。饲粮中植酸盐降低了粘膜匀浆中植酸酶的活性,但这种作用在纯化过程中并不明显。在无菌肠黏膜中,电泳图谱显示植酸酶活性的两个峰,它们对二价金属离子的需求量不同。其中一种纯化的植酸酶不具有任何碱性磷酸酶活性。
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Effects of Intestinal Microflora and Dietary Phytate on Intestinal Phytase Activity in Germfree and Conventionalized Rats
Germfree and conventionalized rats were fed a basal or 2% phytate diet. On mucosal phytase activity, the intestinal microflora has no marked effect. Dietary phytate decreased phytase activity in mucosal homogenates, but this effect was not clear in the course of purification. In the germfree intestinal mucosa, the electrophoretic pattern showed two peaks of phytase activity which were different in divalent metal ion requirements. One of these purified phytases did not have any alkaline phosphatase activity.
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