Application of Microsatellite Markers for Identification of Wine Grape Varieties in Thailand

The use of Microsatellite markers for identifying grapevine varieties. Eighteen primer pairs were selected for identifying amplify 29 grapevine varieties. The result shower that a total of 133 alleles with an average of 7.4 alleles per locus were found to be polymorphic. All alleles size were approximately 112-262 base pair. The cluster analysis using unweighted pair group method with arithmetic mean (UPGMA) based on genetic dissimilarity matrix with 0.9. Twenty nine grapevine varieties were clustered into two major groups. This research indicated that the microsatellite markers could be used to examine and compare the genetic resources among closely samples. The microsatellite maker is useful for discrimination and analysis of genetic diversity of grapevine varieties, and also for grapevine breeding in the future.