Structural identification of the novel 3 way-junction motif

Abstract A novel RNA motif was identified based on its sequence by computational structure modeling. The RNA molecule was reported to be a substrate for the structurally specific endoribonuclease, Dicer, which cleaves doublestranded RNA and cuts out 20−25 nucleotide fragments. This enzymatic property was essential for the potential utilization of the motif in the nanoparticle design of further biological experiments. Herein, the protocol for the prediction of the structure of this motif in-silico is presented, starting from its primary sequence and proceeding through secondary and tertiary structure predictions. Applying RNA architectonics, this novel structural motif, 3wj-nRA, was used for rational RNA nanoparticle design. The molecules, which are based on this three-way junction fold, may assemble into more complex, triangular shaped nano-objects. This trimeric nanoparticle containing 3wj-nRA motif can be further utilized for functionalization and application.