提高植物细胞培养紫杉醇产量包括增加水不混溶的1-丁基-1-甲基吡啶双(三氟甲烷磺酰基)亚胺的用量

IF 0.6 4区工程技术 Q4 CHEMISTRY, MULTIDISCIPLINARY Solvent Extraction Research and Development, Japan Pub Date : 2021-01-01 DOI:10.15261/SERDJ.28.109

Shinjiro Yamamoto, Yumi Kamikubo, Hiroki Tanoue, Michinari Iwasaki, S. Hayashi, H. Miyasaka

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引用次数: 0

摘要

我们在此报告了增加水不混溶离子液体(IL)， 1-丁基-1-甲基吡啶二(三氟甲磺酰基)亚胺(P14-TFSI)的量，在植物细胞培养的水培养基中原位提取紫杉醇的产量。含10 vol% IL的紫杉醇产量是含2.5 vol% IL的紫杉醇产量的1.5倍。此外，在含有10% P14-TFSI的培养物中添加茉莉酸甲酯(MJ)可以提高紫杉醇的产量。加入1µM MJ和10 vol% P14-TFSI的培养液中紫杉醇的含量是不加入P14-TFSI和MJ的对照培养液的78倍以上，是单独加入2.5 vol% P14-TFSI培养液的2倍以上。

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Enhancement of Paclitaxel Production in Plant Cell Culture Including Increased Amount of Water-immiscible 1-Butyl-1-methylpyrrolidinium Bis(trifluoromethanesulfonyl)imide

We report here the effect of increased amount of a water-immiscible ionic liquid (IL), 1-butyl-1-methylpyrrolidinium bis(trifluoromethanesulfonyl)imide (P14-TFSI), enhancing the production amount of paclitaxel with in situ extraction from an aqueous medium in a plant cell culture. The productivity of paclitaxel in the culture including the IL of 10 vol% was 1.5 times greater than that of 2.5 vol%. Moreover, addition of an elicitor, methyl jasmonate (MJ) enhanced the production amount of paclitaxel in the culture including 10% P14-TFSI. The amount of paclitaxel in the culture including 1 µM MJ and 10 vol% P14-TFSI was more than 78 times greater than those in the control culture without both P14-TFSI and MJ, and two times greater than those in the culture including 2.5 vol% P14-TFSI alone.

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来源期刊

Solvent Extraction Research and Development, Japan 工程技术-工程：化工

CiteScore

1.30

自引率

14.30%

发文量

审稿时长

1 months

期刊介绍： Solvent Extraction Research and Development, Japan (Solvent Extr. Res. Dev., Jpn.) is a periodical issued from Japan Association of Solvent Extraction (JASE) containing papers dealing with all aspects of solvent extraction and their related methods, underlying principles, and materials. Original articles, notes, technical reports, and critical reviews will be considered for publication. Original articles must be of reasonably broad scope and significance to the solvent extraction. Notes will originally describe novel work of a limited nature or especially significant work in progress. Authors can contribute some information of novel techniques, equipment or apparatus, reagents and diluents for solvent extraction as technical reports. Critical reviews will be received from the authors to whom the editorial committee asked to contribute.