系统臭氧治疗对大鼠组织再生和炎症反应的生物调节剂作用

J. R. Pires, A. M. Karam, V. Garcia, F. S. Ribeiro, A. E. Pontes, C. R. Andrade, E. C. Zuza
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引用次数: 0

摘要

在臭氧疗法的治疗效果中,细胞代谢和外周组织氧合的改善已被强调。目的探讨臭氧气体生物氧化治疗对创面愈合的全身性影响。材料与方法在24只雄性Wistar大鼠背部皮肤上诱发周长为1.0 cm的组织病变。随机分为两组:1)C组(对照组;n=12):模拟臭氧气体经直肠的应用;2)O3组(试验;n=12):通过直肠注入浓度为50µg/mL的臭氧气体。在第7天和第15天对动物实施安乐死,取出样本,用福尔马林固定,并进行宏观、组织学和组织计量学分析。结果O3组动物在第7天出现混合性炎症,在第15天变为无炎症。C组在第7天出现急性炎症,由急性炎症转变为慢性炎症,从第7天到第15天,慢性炎症明显增加。结果表明,与C组相比,O3组在第7天创面收缩更大(P<0.05),新生血管形成程度更大(P<0.05)。第15天,O3组和C组均表现为完全的再上皮化,而O3组表现为完全的肌肉再生。结论全身臭氧治疗具有生物调节作用,可减轻大鼠皮肤急性炎症特征,促进组织修复和再生。
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Effect of systemic ozone therapy as a biomodulator of tissue regeneration and inflammatory response in rats
Abstract Introduction Among the therapeutic effects of ozone therapy, improvement in cell metabolism and peripheral tissue oxygenation have been highlighted. Objective to evaluate the systemic effect of bio-oxidative therapy with ozone gas on wound healing. Material and method Tissue lesions with a circumference of 1.0 cm were induced in the skin on the back of 24 male Wistar rats. The animals were randomly divided into two groups: 1) Group C (control; n=12): with simulation of the application of ozone gas via the rectum and, 2) Group O3 (test; n=12): with application of ozone gas by means of rectal insufflation at a concentration of 50 µg/mL. The animals were euthanized at seven and 15 days, and samples were removed, fixed in formalin, and submitted to macroscopic, histological, and histometric analyses. Result The animals in the O3 group presented mixed inflammation at seven days, which translated into an absence of inflammation at 15 days. The C group exhibited acute inflammation on the 7th day, translating to chronic inflammation, which significantly increased from the 7th to the 15th day. The findings showed that the O3 group presented greater wound contraction (P<0.05) and a greater degree of neovascularization on the 7th day (P<0.05) when compared to group C. On the 15th day, both groups (O3 and C) showed complete re-epithelialization, however, the O3 group demonstrated complete muscle regeneration. Conclusion The systemic ozone therapy had a biomodulatory effect, reducing the characteristics of acute inflammation and increasing tissue repair and regeneration in rat skin.
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