异质免疫测定声学装置的数值表征和优化

IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION Microfluidics and Nanofluidics Pub Date : 2023-09-16 DOI:10.1007/s10404-023-02676-x
Yingqi Meng, Yupan Wu
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引用次数: 0

摘要

在2019冠状病毒病流行的背景下,加强分析物到传感器表面的运输对于快速检测生物分子至关重要,因为低扩散系数等常见条件会导致检测时间过长。基于saw的方法具有传播损耗小、功耗低、易于集成等优点。然而,气泡诱导声流混合器的微流效应并不稳定和可预测。目前迫切需要开发高效、稳健的声学设备来增强免疫分析。在此,我们利用双SAW流来增强目标分子与固定抗体区域的连续和非侵入性混合。利用声流来搅拌微腔内的流场,加速分析物向功能化表面的传输,同时最大限度地减少局部目标损耗。通过仿真,研究了反应面位置、腔室高度、激发频率、外加电压、抗体浓度和反应速率对微流控芯片结合性能的影响,提出了基于免疫分析增强的微流控芯片优化设计方案。据作者所知,这是第一次通过模拟优化关键参数来增强基于saw的设备的免疫测定的研究。因此,可以增强传感器与靶标的相互作用,同时将非特异性分子从反应表面移出。目前的声流辅助免疫分析技术还可以扩展到其他蛋白质、DNA和细胞分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Numerical characterization and optimization of the acoustic device for heterogeneous immunoassays

In the context of the COVID-19 epidemic, enhancing the transport of analyte to a sensor surface is crucial for rapid detection of biomolecules, since common conditions including low diffusion coefficients cause inordinately long detection times. SAW-based method owns low propagation loss, low power consumption, and ease of integration. However, the microstreaming effect is not stable and predictable using the bubble-induced acoustofluidic mixers. There is a strong need for developing efficient and robust acoustic devices for enhancing immunoassays. We herein take advantage of dual SAW streaming flow to enhance a continuous and non-invasive mixing of the target molecule with the immobilized antibody region. Acoustic streaming flow is utilized to stir the flow field in the micro-chamber, accelerate the transport of analyte to the functionalized surface and simultaneously minimize the localized target depletion. Using simulations, an optimized design of the proposed microfluidic chip is proposed based on the immunoassay enhancement by investigating the influences of the position of the reaction surface, the chamber height, the excitation frequency, the applied voltage, the antibody concentration, and the reaction rate on the binding performance. To the best of authors’ knowledge, it is the first investigation of enhancing immunoassays in SAW-based devices by optimizing the key parameters using simulations. As a result, the sensor target interaction can be enhanced and the nonspecific molecules can be simultaneously displaced from the reaction surface. The current Acoustic streaming flow assisted immunoassay technology can also be extended to other proteins, DNA and cell analysis.

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来源期刊
Microfluidics and Nanofluidics
Microfluidics and Nanofluidics 工程技术-纳米科技
CiteScore
4.80
自引率
3.60%
发文量
97
审稿时长
2 months
期刊介绍: Microfluidics and Nanofluidics is an international peer-reviewed journal that aims to publish papers in all aspects of microfluidics, nanofluidics and lab-on-a-chip science and technology. The objectives of the journal are to (1) provide an overview of the current state of the research and development in microfluidics, nanofluidics and lab-on-a-chip devices, (2) improve the fundamental understanding of microfluidic and nanofluidic phenomena, and (3) discuss applications of microfluidics, nanofluidics and lab-on-a-chip devices. Topics covered in this journal include: 1.000 Fundamental principles of micro- and nanoscale phenomena like, flow, mass transport and reactions 3.000 Theoretical models and numerical simulation with experimental and/or analytical proof 4.000 Novel measurement & characterization technologies 5.000 Devices (actuators and sensors) 6.000 New unit-operations for dedicated microfluidic platforms 7.000 Lab-on-a-Chip applications 8.000 Microfabrication technologies and materials Please note, Microfluidics and Nanofluidics does not publish manuscripts studying pure microscale heat transfer since there are many journals that cover this field of research (Journal of Heat Transfer, Journal of Heat and Mass Transfer, Journal of Heat and Fluid Flow, etc.).
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