预处理对三种兰属植物萌发的影响

IF 1.1 4区 农林科学 Q3 AGRONOMY Journal of Seed Science Pub Date : 2023-04-14 DOI:10.1590/2317-1545v45266264
Milena Cristina Moraes, M. R. Bertão, C. C. Custódio, N. B. Machado-Neto
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引用次数: 0

摘要

摘要/ Abstract摘要:非共生发芽技术在仙人掌属植物中获得了成功。文化媒介的多样化构成是至关重要的;然而,非共生萌发需要有活力的种子。四氮唑试验是评价活力的可靠方法。四氮唑试验效率的决定性步骤是用蔗糖溶液对种子进行预处理,这有可能提高萌发率。因此,本研究旨在评价三种猫尾草属植物的种子在培养基上的萌发情况,培养基上有蔗糖溶液预处理和未加蔗糖溶液预处理。植物的种子,Ctsm。galium和Ctsm。采用四氮唑试验对扁体进行评价,并在两种不同培养基上发芽,用10%蔗糖溶液预处理或不预处理。统计分析表明,使用蔗糖预处理显著提高了该物种的发芽率。结果表明,无论何种菌种和培养基,10%蔗糖预处理24 h均能显著提高Ctsm的发芽率。complanatum Ctsm。galium和Ctsm。osculatum种子。
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The influence of pre-treatment on germination of three species of Catasetum (Orchidaceae)
Abstract: Asymbiotic germination techniques are successful in species of the Catasetum genus. The diverse composition of the culture media can be critical; however, viable seeds are required for asymbiotic germination. The reliable method for viability assessment is the tetrazolium test. A decisive step in the efficiency of the tetrazolium test is the seeds preconditioning with sucrose solution, which has the potential to improve germination. Therefore, this work aimed to evaluate the seed germination of three species of the genus Catasetum in culture media, preconditioned or not with sucrose solution. Seeds of the species Catasetum osculatum, Ctsm. galeritum and Ctsm. complanatum were assessed by tetrazolium test and germinated in two different media, pretreated or not with 10% sucrose solution. The statistical analysis showed that the use of sucrose pretreatment significantly increased the germination rates of the species. We conclude that the pretreatment with 10% sucrose for 24 hours, regardless of the species and the culture medium, significantly increases the germination of Ctsm. complanatum, Ctsm. galeritum and Ctsm. osculatum seeds.
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来源期刊
Journal of Seed Science
Journal of Seed Science Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
2.00
自引率
30.00%
发文量
28
审稿时长
12 weeks
期刊介绍: From 2017 the Journal of Seed Science (JSS) will circulate online version only. Original scientific studies and communications, not yet published or submitted to another journal for publication and written in Portuguese or English, will be accepted for publication. For manuscripts submitted in English, the authors should provide an adequated version. The SCIENTIFIC COMMUNICATION is a category of scientific manuscript which describes a technique, an equipment, new species or observations and surveys of limited results. It has the same scientific rigor as the “Scientific Articles” and the same value as a publication. The classification of a manuscript as a SCIENTIFIC COMMUNICATION is based on its content and scientific merit but it can be a preliminary study, simple and not definitive on a certain subject, with publication justified by its uniqueness and contribution to the area. The Editorial Board of the JSS may invite leading authors of recognized reputation to compose specific Review Articles covering topics of their specialization that will convey to the scientific community the state-of-the-art knowledge related to the specific theme.
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