纸卷加蛭石(PR+V)处理玉米种子发芽试验方法的验证

IF 1.1 4区 农林科学 Q3 AGRONOMY Journal of Seed Science Pub Date : 2023-08-18 DOI:10.1590/2317-1545v45274506
D. K. Rocha, E. R. Carvalho, R. R. Lima, Amanda Carvalho Penido Nardelli, Thaís de Andrade, M. Carvalho, Nilceli Fernandes
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引用次数: 0

摘要

摘要:巴西种子商业化标准要求进行发芽试验。使用一种适当的方法来表达种子的真实生理质量,并尽量减少化学品可能造成的植物毒性,这对生产链很重要。在这种情况下,目的是评估和验证纸卷加蛭石(PR+V)发芽试验方法在玉米种子中的应用。选用两种不同活力水平和不同化学处理条件下对种子处理敏感和耐受的杂交种。选择了10个获得农业部Pecuária (MAPA)认可的实验室进行PR+V发芽试验并对其进行评估。对数据进行统计处理,以估计重复性和再现性,并检查试验的准确性和稳健性。数据在1%和5%的临界范围内是准确、稳健和精确的。对于PR+V方法评估的正常幼苗,曼德尔试验的重复性、再现性和边界的统计结果在1%和5%的临界极限下也是准确和精确的。因此,该方法可常规用于检测玉米种子,特别是用植物检疫产品处理过的玉米种子。
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Validation of the paper roll plus vermiculite (PR+V) germination test methodology for treated corn seeds
Abstract: A germination test is required by Brazilian standards for seed commercialization. The use of an adequate methodology that expresses the real physiological quality of seeds and minimizes possible phytotoxicity by chemicals is important for the production chain. In this context, the aim was to evaluate and validate the paper roll plus vermiculite (PR+V) germination test methodology for use in corn seeds. Two hybrids, one sensitive and the other tolerant to seed treatment, both with different vigor levels and under different chemical treatments, were used. Ten laboratories accredited by the Ministério da Agricultura e Pecuária (MAPA) were selected to perform and evaluate the PR+V germination tests. The data were subjected to statistical procedures to estimate the repeatability and reproducibility and check the accuracy and robustness of the test. The data were accurate, robust, and precise within the critical limits of 1 and 5%. The statistical results for repeatability, reproducibility, and bounds of the Mandel test were also accurate and precise at the critical limits of 1 and 5% for the normal seedlings evaluated by the PR+V methodology. Thus, the methodology can be used routinely to test corn seeds, especially those treated with phytosanitary products.
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来源期刊
Journal of Seed Science
Journal of Seed Science Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
2.00
自引率
30.00%
发文量
28
审稿时长
12 weeks
期刊介绍: From 2017 the Journal of Seed Science (JSS) will circulate online version only. Original scientific studies and communications, not yet published or submitted to another journal for publication and written in Portuguese or English, will be accepted for publication. For manuscripts submitted in English, the authors should provide an adequated version. The SCIENTIFIC COMMUNICATION is a category of scientific manuscript which describes a technique, an equipment, new species or observations and surveys of limited results. It has the same scientific rigor as the “Scientific Articles” and the same value as a publication. The classification of a manuscript as a SCIENTIFIC COMMUNICATION is based on its content and scientific merit but it can be a preliminary study, simple and not definitive on a certain subject, with publication justified by its uniqueness and contribution to the area. The Editorial Board of the JSS may invite leading authors of recognized reputation to compose specific Review Articles covering topics of their specialization that will convey to the scientific community the state-of-the-art knowledge related to the specific theme.
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