酶光度动力学法测定不同剂型苯扎氯铵的应用

IF 0.7 Q4 CHEMISTRY, ANALYTICAL Methods and Objects of Chemical Analysis Pub Date : 2023-01-01 DOI:10.17721/moca.2023.5-12
M. Blazheyevskіy, O. Koval’ska
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引用次数: 0

摘要

介绍了一种准确灵敏地测定苯扎氯铵的动力学光度法。该方法以乙酰胆碱酯酶反应抑制乙酰胆碱酶解为基础。通过酶促反应的抑制程度来确定苯扎氯铵的用量,并通过剩余未反应底物乙酰胆碱来评价其抑制程度。反应混合物中乙酰胆碱残留量的测定采用动力学光度法,利用对非尼替丁与过氧乙酸的指示剂氧化反应,该反应是在反应混合物中加入过量的过氧化氢一段时间的过水解辅助反应过程中形成的。抑制度-浓度曲线在1.4∙10-6 ~ 7.0∙10-6 mol/ L范围内呈线性关系,相关系数为0.998。LOQ为1.9∙10-6 mol/L。对不同的实验参数pH、缓冲液进行了仔细的研究和优化。所提出的方法已成功地应用于药物制剂。将结果与已建立的方法进行统计比较,结果非常一致,证明在准确度和精密度上没有显著差异。“Virotec-intim”制剂的RSD为3.2% (δ*=- 0.3%)。
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Application of Enzymatic Photometric Kinetic Method for Determination of Benzalkonium Chloride in Various Dosage Forms
A kinetic-photometric method for accurate and sensitive determination of benzalconium chloride has been described. The method is based on inhibition of enzymatic hydrolysis of acetylcholine by enzyme acethylcholinestherase reaction. The amount of benzalconium chloride was determined by the degree of inhibition of the enzymatic reaction, which was evaluated by the residual unreacted substrate - acetylcholine. Determination of the residual amount of acetylcholine in the reaction mixture was performed by a kineticphotometric method using an indicator oxidation reaction of p-phenetidine with peracetic acid, which is formed during the auxiliration reaction of perhydrolysis with addition of excess hydrogen peroxide in the reaction mixture over a period of time. The inhibition degree –concentration plot is linear over the range of 1.4 ∙10-6 – 7.0∙10-6 mol/ L with correlation coefficient of 0.998. The LOQ was 1.9∙10-6 mol/L. The different experimental parameters pH, buffer solution was carefully studied and optimized. The proposed method has been successfully applied to pharmaceutical formulations. Statistical comparison of the results with a good established reported method showed excellent agreement and proved that there is no significant difference in the accuracy and precision. For “Virotec-intim” preparation RSD was 3.2 % (δ*=- 0.3 %).
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来源期刊
CiteScore
1.00
自引率
14.30%
发文量
12
期刊介绍: The journal "Methods and objects of chemical analysis" is peer-review journal and publishes original articles of theoretical and experimental analysis on topical issues and bio-analytical chemistry, chemical and pharmaceutical analysis, as well as chemical metrology. Submitted works shall cover the results of completed studies and shall make scientific contributions to the relevant area of expertise. The journal publishes review articles, research articles and articles related to latest developments of analytical instrumentations.
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