{"title":"从海藻废弃物中分离出来的细菌中产生和分解酶酶","authors":"Isna Rahma Dini, Kampus Bina Widya, Balai Besar","doi":"10.17969/JTIPI.V6I3.2315","DOIUrl":null,"url":null,"abstract":"Seaweed waste is a source of bacteria that can produce cellulase enzyme. PMP 0126w isolate is one collection isolate of BBP4BKP obtained from seaweed Glacilaria sp. waste from Pameungpeuk area, Garut, West Java. The aims of this research were to produce and characterize the cellulase enzyme. PMP 0126w isolate was a Gram-positive rod shape bacteria. The isolate had 0.8 cellulolytic index on Carboxymethyl Cellulose (CMC) agar medium. The highest cellulase activity obtained on the third day of fermentation time with a cellulase activity of 0,074 U/mL and specific activity of 0.092 U/mg. Optimum activity of the cellulase enzyme crude extract was pH 5 and 300C. The activity of the cellulase was increased by addition of 5 mM CaCl2 and 10 mM FeCl3 ions and decreased by the addition of 10 mM ZnCl2 ions. The cellulase with the highest activity of 0.237 U/mL was obtained from Glacilaria sp. seaweed waste treated with NaOH 6%. Keywords: cellulose, characterization, production, seaweed waste","PeriodicalId":31317,"journal":{"name":"Jurnal Teknologi dan Industri Pertanian Indonesia","volume":"1 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2014-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"Produksi dan Karakterisasi Enzim Selulase Ekstrak Kasar dari Bakteri yang Diisolasi dari Limbah Rumput Laut\",\"authors\":\"Isna Rahma Dini, Kampus Bina Widya, Balai Besar\",\"doi\":\"10.17969/JTIPI.V6I3.2315\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Seaweed waste is a source of bacteria that can produce cellulase enzyme. PMP 0126w isolate is one collection isolate of BBP4BKP obtained from seaweed Glacilaria sp. waste from Pameungpeuk area, Garut, West Java. The aims of this research were to produce and characterize the cellulase enzyme. PMP 0126w isolate was a Gram-positive rod shape bacteria. The isolate had 0.8 cellulolytic index on Carboxymethyl Cellulose (CMC) agar medium. The highest cellulase activity obtained on the third day of fermentation time with a cellulase activity of 0,074 U/mL and specific activity of 0.092 U/mg. Optimum activity of the cellulase enzyme crude extract was pH 5 and 300C. The activity of the cellulase was increased by addition of 5 mM CaCl2 and 10 mM FeCl3 ions and decreased by the addition of 10 mM ZnCl2 ions. The cellulase with the highest activity of 0.237 U/mL was obtained from Glacilaria sp. seaweed waste treated with NaOH 6%. Keywords: cellulose, characterization, production, seaweed waste\",\"PeriodicalId\":31317,\"journal\":{\"name\":\"Jurnal Teknologi dan Industri Pertanian Indonesia\",\"volume\":\"1 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2014-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Jurnal Teknologi dan Industri Pertanian Indonesia\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.17969/JTIPI.V6I3.2315\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Jurnal Teknologi dan Industri Pertanian Indonesia","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17969/JTIPI.V6I3.2315","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
摘要
海藻废料是能产生纤维素酶的细菌的来源。pmp0126w分离物是从西爪哇加鲁特paameungpeuk地区的海藻Glacilaria sp.废物中获得的BBP4BKP收集分离物。本研究的目的是生产和表征纤维素酶。pmp0126w分离株为革兰氏阳性杆状菌。该分离物在羧甲基纤维素(CMC)琼脂培养基上的纤维素分解指数为0.8。发酵第3天纤维素酶活性最高，为0.074 U/mL，比活性为0.092 U/mg。纤维素酶粗提物的最佳活性为pH 5和300C。添加5 mM CaCl2和10 mM FeCl3离子可提高纤维素酶的活性，添加10 mM ZnCl2离子可降低纤维素酶的活性。以6% NaOH处理的Glacilaria sp.海藻废料为原料，纤维素酶活性最高，为0.237 U/mL。关键词:纤维素;表征;生产
Produksi dan Karakterisasi Enzim Selulase Ekstrak Kasar dari Bakteri yang Diisolasi dari Limbah Rumput Laut
Seaweed waste is a source of bacteria that can produce cellulase enzyme. PMP 0126w isolate is one collection isolate of BBP4BKP obtained from seaweed Glacilaria sp. waste from Pameungpeuk area, Garut, West Java. The aims of this research were to produce and characterize the cellulase enzyme. PMP 0126w isolate was a Gram-positive rod shape bacteria. The isolate had 0.8 cellulolytic index on Carboxymethyl Cellulose (CMC) agar medium. The highest cellulase activity obtained on the third day of fermentation time with a cellulase activity of 0,074 U/mL and specific activity of 0.092 U/mg. Optimum activity of the cellulase enzyme crude extract was pH 5 and 300C. The activity of the cellulase was increased by addition of 5 mM CaCl2 and 10 mM FeCl3 ions and decreased by the addition of 10 mM ZnCl2 ions. The cellulase with the highest activity of 0.237 U/mL was obtained from Glacilaria sp. seaweed waste treated with NaOH 6%. Keywords: cellulose, characterization, production, seaweed waste
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