{"title":"文章标题贵州藜属植物遗传多样性及分化的探索","authors":"Rongrong Wang","doi":"10.17582/journal.pjz/20221016141056","DOIUrl":null,"url":null,"abstract":"To understand the genetic diversity and genetic differentiation status of Craspedacusta sowerbyi Lankester, 1880 in Guizhou Province, genetic diversity and genetic structure analyses of three geographical populations of C. sowerbyi in Guizhou Province were performed using the results of PCR amplification and direct sequencing based on the mitochondrial DNA COI gene. Sixty-two individuals displayed four haplotypes, and the overall haplotype diversity (Hd) and nucleotide diversity (Pi) of the three C. sowerbyi groups were 0.531 and 0.00067, respectively. Analysis of molecular variance (AMOVA) showed that inter- and intrapopulation variation accounted for 41.63039% and 58.36961%, respectively, of the total variation, and the genetic variation coefficient (F st ) was 0.05268. The gene flow (Nm) values between the three C. sowerbyi populations were <1. The results of neutrality, Fu’s Fs and Tajima’s D tests did not show significant negative values. The overall nucleotide diversity of the three C. sowerbyi populations in Guizhou Province was low, especially in the Xiuwen County population, the genetic variation mainly occurred within the populations.","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":null,"pages":null},"PeriodicalIF":0.5000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Exploration of the Genetic Diversity and Differentiation of Craspedacusta sowerbyi in Guizhou Province\",\"authors\":\"Rongrong Wang\",\"doi\":\"10.17582/journal.pjz/20221016141056\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"To understand the genetic diversity and genetic differentiation status of Craspedacusta sowerbyi Lankester, 1880 in Guizhou Province, genetic diversity and genetic structure analyses of three geographical populations of C. sowerbyi in Guizhou Province were performed using the results of PCR amplification and direct sequencing based on the mitochondrial DNA COI gene. Sixty-two individuals displayed four haplotypes, and the overall haplotype diversity (Hd) and nucleotide diversity (Pi) of the three C. sowerbyi groups were 0.531 and 0.00067, respectively. Analysis of molecular variance (AMOVA) showed that inter- and intrapopulation variation accounted for 41.63039% and 58.36961%, respectively, of the total variation, and the genetic variation coefficient (F st ) was 0.05268. The gene flow (Nm) values between the three C. sowerbyi populations were <1. The results of neutrality, Fu’s Fs and Tajima’s D tests did not show significant negative values. The overall nucleotide diversity of the three C. sowerbyi populations in Guizhou Province was low, especially in the Xiuwen County population, the genetic variation mainly occurred within the populations.\",\"PeriodicalId\":19975,\"journal\":{\"name\":\"Pakistan Journal of Zoology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.5000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pakistan Journal of Zoology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.17582/journal.pjz/20221016141056\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"ZOOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pakistan Journal of Zoology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.17582/journal.pjz/20221016141056","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ZOOLOGY","Score":null,"Total":0}
Exploration of the Genetic Diversity and Differentiation of Craspedacusta sowerbyi in Guizhou Province
To understand the genetic diversity and genetic differentiation status of Craspedacusta sowerbyi Lankester, 1880 in Guizhou Province, genetic diversity and genetic structure analyses of three geographical populations of C. sowerbyi in Guizhou Province were performed using the results of PCR amplification and direct sequencing based on the mitochondrial DNA COI gene. Sixty-two individuals displayed four haplotypes, and the overall haplotype diversity (Hd) and nucleotide diversity (Pi) of the three C. sowerbyi groups were 0.531 and 0.00067, respectively. Analysis of molecular variance (AMOVA) showed that inter- and intrapopulation variation accounted for 41.63039% and 58.36961%, respectively, of the total variation, and the genetic variation coefficient (F st ) was 0.05268. The gene flow (Nm) values between the three C. sowerbyi populations were <1. The results of neutrality, Fu’s Fs and Tajima’s D tests did not show significant negative values. The overall nucleotide diversity of the three C. sowerbyi populations in Guizhou Province was low, especially in the Xiuwen County population, the genetic variation mainly occurred within the populations.
期刊介绍:
Pakistan Journal of Zoology (Pakistan J. Zool.) publishes original articles in English on all aspects of animal life. Generally these articles will be in, or related to one of the following subject areas: Physiology, Cell Biology, Molecular Biology, Genetics, Bioinformatics, Toxicology, Forensic Science, Developmental Biology, Entomology, Parasitology, Microbiology, Biotechnology, Pathology, Palaeontology. Taxonomy, Environmental Biology, Wildlife, Fisheries, Vertebrate and Invertebrate Morphology. Additionally, the journal considers research on health and clinical studies. Short communications are regularly considered, however, uninvited review articles, first records/reports of known species, case reports/studies and survey reports are not published in Pakistan Journal of Zoology.