评估水厌氧介质中微生物矿化率的简单方法

Q2 Environmental Science Acta Limnologica Brasiliensia Pub Date : 2021-01-01 DOI:10.1590/s2179-975x2721
M. B. Cunha-Santino, I. Bianchini Júnior
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引用次数: 0

摘要

摘要:本研究的目的是提出并讨论一种简单的压力测量方法来量化有机资源(如叶片、细枝和大型植物碎屑)的微生物厌氧矿化产生的气体排放率。该方法可在实验室条件下使用。该方法包括使用附在反应烧瓶上的水压计。以帕那帕纳帕内玛河(Paranapanema River)的水和霸王泰法(Typha domingensis)为样品进行孵育,实验时间为9.8个月。详细介绍了准备孵育的程序,以及将体积测量转换为碳质量(即每日碳气体排放率)的计算。根据T. domingensis矿化分析的结果,可以证明与矿化有关的许多事件可以得到充分的解决(例如,非均质碎屑组成)。该方法与成矿第30天之后的动力学实验结果(作为参考)较为接近,表明该方法主要用于中长期实验。正如多根氏滴虫孵育的例子所示,这种方法对于几种有机资源矿化的系统比较和所涉及的主要参数(如反应速率常数)的初步测量特别有价值。该方法与其他短期实验相结合,可以大大提高对水生环境中有机资源循环的认识。
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Simple methodological approach for assessing microbial mineralization rates in an aqueous anaerobic medium
Abstract: The aim of this study was to propose and discuss a simple manometric method to quantify the emission rates of gases resulting from the microbial anaerobic mineralization of organic resources, as leaves, thin branches, and macrophyte detritus. The proposed method can be used under laboratory conditions. The method consists of using a water pressure gauge attached to the reaction flask. The incubations were prepared with samples of water from Paranapanema River and Typha domingensis, the experiment lasted 9.8 months. The procedures for preparing the incubations are presented in detail, as well as the calculations for the conversion of the volumetric measurement into carbon mass (i.e., daily rate of carbon gas emissions). According to the results obtained from T. domingensis mineralization assays it was possible to demonstrate that, numerous events related to mineralization could be adequately addressed (e.g., the heterogeneous detritus composition). The results of this method were quite convergent with those obtained in kinetic experiments (used as a reference) after the 30th mineralization day, suggesting the use of this method mainly for medium- and long-term experiments. As exemplified by T. domingensis incubations, this method is particularly valuable for the systemic comparison of the several organic resources mineralization and for the primary measurement of the main parameters involved (e.g., reaction rates constants). This method combined with other short-term experiments can greatly improve the understanding of the cycling of organic resources in aquatic environments.
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来源期刊
Acta Limnologica Brasiliensia
Acta Limnologica Brasiliensia Environmental Science-Ecology
CiteScore
2.20
自引率
0.00%
发文量
17
审稿时长
50 weeks
期刊最新文献
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