人类脐带不同区域间质群体的表型和分化潜力综述

M. Conconi, R. Liddo, M. Tommasini, C. Calore, P. Parnigotto
{"title":"人类脐带不同区域间质群体的表型和分化潜力综述","authors":"M. Conconi, R. Liddo, M. Tommasini, C. Calore, P. Parnigotto","doi":"10.2174/1875043501104010006","DOIUrl":null,"url":null,"abstract":"Fibroblast-like cells with properties similar to mesenchymal stromal cells (MSCs) are present in human umbilical cord (hUC). In accordance with the international minimal criteria for defining multipotent mesenchymal stromal cells, hUC cells are designed MSCs being plastic adherent, positive to specific non hematopoietic lineage biomarkers, able to be both in vitro long term cultured and differentiated into osteoblasts, chondroblasts and adipocytes. In this review, a panoramic view of phenotypic characteristics of hUC cells derived from various UC parts are described. The high heterogeneity of extraction, culture and analysis procedures hinder the ability to precisely identify UC stromal cells. As a result, different phenotypic profiles are detectable not only among the cells obtained from the various parts of cord, but also inside the same UC regions, suggesting that UC-MSCs may represent an unique cell family whose components present various degree of stemness. However, in vitro and in vivo evidence indicates Wharton's jelly as the best source of MSCs, because its cells present a wide range of potential therapeutic applications.","PeriodicalId":88761,"journal":{"name":"The open tissue engineering and regenerative medicine journal","volume":"4 1","pages":"6-20"},"PeriodicalIF":0.0000,"publicationDate":"2011-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"74","resultStr":"{\"title\":\"Phenotype and Differentiation Potential of Stromal Populations Obtained from Various Zones of Human Umbilical Cord: An Overview\",\"authors\":\"M. Conconi, R. Liddo, M. Tommasini, C. Calore, P. Parnigotto\",\"doi\":\"10.2174/1875043501104010006\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Fibroblast-like cells with properties similar to mesenchymal stromal cells (MSCs) are present in human umbilical cord (hUC). In accordance with the international minimal criteria for defining multipotent mesenchymal stromal cells, hUC cells are designed MSCs being plastic adherent, positive to specific non hematopoietic lineage biomarkers, able to be both in vitro long term cultured and differentiated into osteoblasts, chondroblasts and adipocytes. In this review, a panoramic view of phenotypic characteristics of hUC cells derived from various UC parts are described. The high heterogeneity of extraction, culture and analysis procedures hinder the ability to precisely identify UC stromal cells. As a result, different phenotypic profiles are detectable not only among the cells obtained from the various parts of cord, but also inside the same UC regions, suggesting that UC-MSCs may represent an unique cell family whose components present various degree of stemness. However, in vitro and in vivo evidence indicates Wharton's jelly as the best source of MSCs, because its cells present a wide range of potential therapeutic applications.\",\"PeriodicalId\":88761,\"journal\":{\"name\":\"The open tissue engineering and regenerative medicine journal\",\"volume\":\"4 1\",\"pages\":\"6-20\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2011-12-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"74\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The open tissue engineering and regenerative medicine journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2174/1875043501104010006\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The open tissue engineering and regenerative medicine journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/1875043501104010006","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 74

摘要

具有类似间充质间质细胞(MSCs)特性的成纤维细胞样细胞存在于人脐带(hUC)中。根据定义多能间充质基质细胞的国际最低标准,hUC细胞被设计为具有可塑性粘附性的MSCs,对特定的非造血谱系生物标志物呈阳性,能够在体外长期培养并分化为成骨细胞、成软骨细胞和脂肪细胞。在这篇综述中,描述了来自UC不同部位的UC细胞的表型特征的全景视图。提取、培养和分析过程的高度异质性阻碍了精确鉴定UC间质细胞的能力。因此,不仅在脐带不同部位获得的细胞中可以检测到不同的表型谱,而且在相同的UC区域内也可以检测到不同的表型谱,这表明UC- mscs可能代表了一个独特的细胞家族,其成分具有不同程度的干性。然而,体外和体内的证据表明,沃顿水母是间充质干细胞的最佳来源,因为它的细胞具有广泛的潜在治疗应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Phenotype and Differentiation Potential of Stromal Populations Obtained from Various Zones of Human Umbilical Cord: An Overview
Fibroblast-like cells with properties similar to mesenchymal stromal cells (MSCs) are present in human umbilical cord (hUC). In accordance with the international minimal criteria for defining multipotent mesenchymal stromal cells, hUC cells are designed MSCs being plastic adherent, positive to specific non hematopoietic lineage biomarkers, able to be both in vitro long term cultured and differentiated into osteoblasts, chondroblasts and adipocytes. In this review, a panoramic view of phenotypic characteristics of hUC cells derived from various UC parts are described. The high heterogeneity of extraction, culture and analysis procedures hinder the ability to precisely identify UC stromal cells. As a result, different phenotypic profiles are detectable not only among the cells obtained from the various parts of cord, but also inside the same UC regions, suggesting that UC-MSCs may represent an unique cell family whose components present various degree of stemness. However, in vitro and in vivo evidence indicates Wharton's jelly as the best source of MSCs, because its cells present a wide range of potential therapeutic applications.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Restoration of Heart Function Using Transplantation of Human Umbilical Cord Matrix-Derived Cardiomyocytes and Vascular Endothelial Growth Factor Recent advances in derivation of functional hepatocytes from placental stem cells Macrophages Recruitment and Activation by α-gal NanoparticlesAccelerate Regeneration and Can Improve Biomaterials Efficacy in TissueEngineering Biomat _dBase: A Database on Biomaterials Novel immunomodulatory markers expressed by human WJ-MSC: An updated review in regenerative and reparative medicine
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1