LC-MS/MS分析及分子网络技术对梅利蓬青霉类核蛋白氮杂啉类化合物的脱皮作用

IF 1.3 4区 化学 Q3 CHEMISTRY, MULTIDISCIPLINARY Journal of the Brazilian Chemical Society Pub Date : 2023-07-24 DOI:10.21577/0103-5053.20230027
E. Peres, Mayane P. Souza, T. Sousa, C. da Silva, A. Barros, F. D. da Silva, E. Costa, L. D. de Medeiros, M. Forim, A. D. de Souza, Weider H. P. Paz, G. D. da Silva, A. D. de Souza, H. Koolen
{"title":"LC-MS/MS分析及分子网络技术对梅利蓬青霉类核蛋白氮杂啉类化合物的脱皮作用","authors":"E. Peres, Mayane P. Souza, T. Sousa, C. da Silva, A. Barros, F. D. da Silva, E. Costa, L. D. de Medeiros, M. Forim, A. D. de Souza, Weider H. P. Paz, G. D. da Silva, A. D. de Souza, H. Koolen","doi":"10.21577/0103-5053.20230027","DOIUrl":null,"url":null,"abstract":"Penicillium meliponae, a recently described and rare species, was isolated as an endophytic fungus from the Amazonian plant Duguetia sthelechantha, and has been proven to be a pigment producer. Considering the high productivity of this species and the lack of data on its chemical composition, the present study aimed to characterize the chemical profile of P. meliponae and evaluate the influence of agitation and the use of different culture media. For this purpose, liquid chromatography coupled with mass spectrometry (LC-MS/MS) and molecular networking were used, allowing the identification of 17 azaphilone molecules with sclerotiorin-like skeletons, becoming the first chemical report of this species. In addition, the different production patterns in the tested culture media were indicative that this species is sensitive to changes in the composition of the carbon source and to the presence of agitation. Furthermore, this work contributes to the fragmentation mechanisms of the different possible structural arrangements for azaphilones of the sclerotiorin type and serves as a repository of information on the gas-phase behavior of this type of metabolite in mass spectrometry experiments and will assist future studies aimed at the discovery of azaphilones.","PeriodicalId":17257,"journal":{"name":"Journal of the Brazilian Chemical Society","volume":"1 1","pages":""},"PeriodicalIF":1.3000,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Dereplication of Sclerotiorin-Like Azaphilones Produced by Penicillium meliponae Using LC-MS/MS Analysis and Molecular Networking\",\"authors\":\"E. Peres, Mayane P. Souza, T. Sousa, C. da Silva, A. Barros, F. D. da Silva, E. Costa, L. D. de Medeiros, M. Forim, A. D. de Souza, Weider H. P. Paz, G. D. da Silva, A. D. de Souza, H. Koolen\",\"doi\":\"10.21577/0103-5053.20230027\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Penicillium meliponae, a recently described and rare species, was isolated as an endophytic fungus from the Amazonian plant Duguetia sthelechantha, and has been proven to be a pigment producer. Considering the high productivity of this species and the lack of data on its chemical composition, the present study aimed to characterize the chemical profile of P. meliponae and evaluate the influence of agitation and the use of different culture media. For this purpose, liquid chromatography coupled with mass spectrometry (LC-MS/MS) and molecular networking were used, allowing the identification of 17 azaphilone molecules with sclerotiorin-like skeletons, becoming the first chemical report of this species. In addition, the different production patterns in the tested culture media were indicative that this species is sensitive to changes in the composition of the carbon source and to the presence of agitation. Furthermore, this work contributes to the fragmentation mechanisms of the different possible structural arrangements for azaphilones of the sclerotiorin type and serves as a repository of information on the gas-phase behavior of this type of metabolite in mass spectrometry experiments and will assist future studies aimed at the discovery of azaphilones.\",\"PeriodicalId\":17257,\"journal\":{\"name\":\"Journal of the Brazilian Chemical Society\",\"volume\":\"1 1\",\"pages\":\"\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2023-07-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of the Brazilian Chemical Society\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.21577/0103-5053.20230027\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Brazilian Chemical Society","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.21577/0103-5053.20230027","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0

摘要

青霉菌(Penicillium meliponae)是最近发现的一种罕见的内生真菌,它是从亚马逊地区的植物Duguetia stheelechantha中分离出来的,并已被证明是一种色素生产真菌。考虑到该物种的高产和缺乏化学成分的数据,本研究旨在表征P. meliponae的化学特征,并评估搅拌和不同培养基使用的影响。为此,利用液相色谱-质谱联用(LC-MS/MS)和分子网络技术,鉴定了17个具有类似于菌核蛋白骨架的氮唑啉分子,成为该物种的首次化学报道。此外,培养基中不同的生产模式表明,该物种对碳源组成的变化和搅拌的存在很敏感。此外,这项工作有助于研究固结蛋白类型的氮化氮酮的不同可能结构排列的断裂机制,并在质谱实验中为这类代谢物的气相行为提供信息库,并将有助于未来旨在发现氮化氮酮的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Dereplication of Sclerotiorin-Like Azaphilones Produced by Penicillium meliponae Using LC-MS/MS Analysis and Molecular Networking
Penicillium meliponae, a recently described and rare species, was isolated as an endophytic fungus from the Amazonian plant Duguetia sthelechantha, and has been proven to be a pigment producer. Considering the high productivity of this species and the lack of data on its chemical composition, the present study aimed to characterize the chemical profile of P. meliponae and evaluate the influence of agitation and the use of different culture media. For this purpose, liquid chromatography coupled with mass spectrometry (LC-MS/MS) and molecular networking were used, allowing the identification of 17 azaphilone molecules with sclerotiorin-like skeletons, becoming the first chemical report of this species. In addition, the different production patterns in the tested culture media were indicative that this species is sensitive to changes in the composition of the carbon source and to the presence of agitation. Furthermore, this work contributes to the fragmentation mechanisms of the different possible structural arrangements for azaphilones of the sclerotiorin type and serves as a repository of information on the gas-phase behavior of this type of metabolite in mass spectrometry experiments and will assist future studies aimed at the discovery of azaphilones.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
2.90
自引率
7.10%
发文量
99
审稿时长
3.4 months
期刊介绍: The Journal of the Brazilian Chemical Society embraces all aspects of chemistry except education, philosophy and history of chemistry. It is a medium for reporting selected original and significant contributions to new chemical knowledge.
期刊最新文献
Quantitative GC-MS Analysis of Sawdust Bio-Oil A New Molecularly Imprinted Polymer for In-Tube SPME/UHPLC-MS/MS of Anandamide in Plasma Samples Evaluation of Extraction Parameters for the Analysis of Lipid Classes in Plants Polyvinyl Alcohol/Pectin-Based Hydrogel as Sorptive Phase for the Determination of Freely Dissolved Parabens in Urine Samples by LC-DAD Evaluation of Polymer Self-Coating on Aluminized Silica Support as Stationary Phase for High-Performance Liquid Chromatography
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1