Antibiotic Resistance Pattern and Biofilm Formation of Staphylococcus and Enterobacteriaceae Isolates from Clinical Samples of Patients with Urinary Tract and Surgical Site Infections in Kinshasa, Democratic Republic of Congo

of infections is compromised worldwide by that are resistant to multiple antibiotics [2]. Infections caused by multidrug-resistant organisms (MDROs) are associated with Abstract Background: Gram-negative and Gram-positive microorganisms are responsible for both community and hospital acquired infections. The increase, emergence, and spread of antimicrobial resistance among bacteria are the most important health problems worldwide. One of the mechanisms of resistance used by bacteria is biofilm formation. The aim of this study was to investigate the antibiotic resistance pattern and the biofilm formation ability of Staphylococcus aureus and Enterobacteriaceae isolates. Methods : A total of 18 Staphylococcus aureus and 60 Enterobacteriaceae clinical isolates were collected from patients with urinary and surgical site infections in Hôpital Biamba Marie Mutombo and Saint Joseph Hospital. The antibiotic susceptibility profile of the isolates were determined by disk-diffusion method. Microtiter plate method was used to assess the ability of bacteria strains to produce and to form un biofilm. Results : The majority of S. aureus and Enterobacteriacea clinical isolates were highly resistant to the majority of antibiotics and biofilm producers. S. aureus strains were 100 % resistant to ampicillin-sulbactam, piperacillin-tazobactam, vancomycin, amoxicillin-clavulanic acid, levofloxacin, and aztreonam. E. coli, Enterobacter sp. , Citrobacter sp., and Serratia sp. were 100 % resistant to