{"title":"通过护理点COVID-19检测快速简便地检测SARS-CoV-2","authors":"A. Eftekhari, A. Nasibova, R. Khalilov","doi":"10.29228/PROC.78","DOIUrl":null,"url":null,"abstract":"COVID-19 as a public health concern of the world has spread worldwide and a combination of vari-ous methods including tomography imaging, reverse transcription-polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA) and cell culturing were developed to detect and identify SARS-CoV-2. Due to the absence of specific antiviral agents or vaccines for COVID-19 treat-ment, early detection and identification are vital. An alternative, sensitive, fast point-of-care (POC) detection tool that can be routinely used by health care providers utilizing biological fluids as a specimen before starting an emergency process is desired. Efforts are underway to develop more effective diagnostic and surveillance technologies with loop-mediated isothermal amplification (LAMP) tests, antibody testing and microfluidic RT-PCR devices (Lab-on-a-chip). Point-of-care diagnostics are promising candidates in SARS-Cov-2 detection and encourage scientists to improve their technologies beyond conception. The reverse transcription LAMP (RT-LAMP) method developed for SARS-CoV-2 could detect the virus even in saliva samples in less than an hour (Harapan et al., 2020). Lab-on-a-chip devices contain a small size chip, microchannel, microelectrodes and microheater. Cell ly-sis, DNA extraction and PCR amplification stages could be integrated on these microchips (Sharma et al., 2020). Because of the rapid detection, small volume of the specimen and integration with PCR in a portable tiny system, these devices are promising for SARS-CoV-2 detection (Huang et al., 2018). The validity and sensitivity of all the above-mentioned methods need to be improved for salivary specimen usage; in case of improvement, they might provide an opportunity for salivary detection of the virus without a waiting period and complex analytical infrastructure.","PeriodicalId":54068,"journal":{"name":"Proceedings of the Institute of Mathematics and Mechanics","volume":"1 1","pages":""},"PeriodicalIF":0.7000,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Rapid and simple detection of SARS-CoV-2 with point-of-care COVID-19 testing\",\"authors\":\"A. Eftekhari, A. Nasibova, R. Khalilov\",\"doi\":\"10.29228/PROC.78\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"COVID-19 as a public health concern of the world has spread worldwide and a combination of vari-ous methods including tomography imaging, reverse transcription-polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA) and cell culturing were developed to detect and identify SARS-CoV-2. Due to the absence of specific antiviral agents or vaccines for COVID-19 treat-ment, early detection and identification are vital. An alternative, sensitive, fast point-of-care (POC) detection tool that can be routinely used by health care providers utilizing biological fluids as a specimen before starting an emergency process is desired. Efforts are underway to develop more effective diagnostic and surveillance technologies with loop-mediated isothermal amplification (LAMP) tests, antibody testing and microfluidic RT-PCR devices (Lab-on-a-chip). Point-of-care diagnostics are promising candidates in SARS-Cov-2 detection and encourage scientists to improve their technologies beyond conception. The reverse transcription LAMP (RT-LAMP) method developed for SARS-CoV-2 could detect the virus even in saliva samples in less than an hour (Harapan et al., 2020). Lab-on-a-chip devices contain a small size chip, microchannel, microelectrodes and microheater. Cell ly-sis, DNA extraction and PCR amplification stages could be integrated on these microchips (Sharma et al., 2020). Because of the rapid detection, small volume of the specimen and integration with PCR in a portable tiny system, these devices are promising for SARS-CoV-2 detection (Huang et al., 2018). The validity and sensitivity of all the above-mentioned methods need to be improved for salivary specimen usage; in case of improvement, they might provide an opportunity for salivary detection of the virus without a waiting period and complex analytical infrastructure.\",\"PeriodicalId\":54068,\"journal\":{\"name\":\"Proceedings of the Institute of Mathematics and Mechanics\",\"volume\":\"1 1\",\"pages\":\"\"},\"PeriodicalIF\":0.7000,\"publicationDate\":\"2020-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Proceedings of the Institute of Mathematics and Mechanics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.29228/PROC.78\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MATHEMATICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of the Institute of Mathematics and Mechanics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.29228/PROC.78","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATHEMATICS","Score":null,"Total":0}
引用次数: 0
摘要
COVID-19作为全球公共卫生问题已在全球范围内蔓延,并开发了多种方法,包括断层扫描成像、逆转录聚合酶链反应(RT-PCR)、酶联免疫吸附试验(ELISA)和细胞培养,以检测和鉴定SARS-CoV-2。由于缺乏治疗COVID-19的特异性抗病毒药物或疫苗,因此早期发现和识别至关重要。需要一种替代的、灵敏的、快速的即时检测工具,这种工具可以在开始紧急处理之前由卫生保健提供者常规使用,利用生物液体作为标本。人们正在努力开发更有效的诊断和监测技术,包括环介导的等温扩增(LAMP)测试、抗体测试和微流控RT-PCR设备(芯片实验室)。即时诊断是检测SARS-Cov-2的有希望的候选方法,并鼓励科学家在概念之外改进他们的技术。针对SARS-CoV-2开发的逆转录LAMP (RT-LAMP)方法甚至可以在不到一小时的时间内从唾液样本中检测到该病毒(Harapan et al., 2020)。芯片实验室设备包含小尺寸芯片、微通道、微电极和微加热器。细胞裂解、DNA提取和PCR扩增阶段可以集成在这些微芯片上(Sharma et al., 2020)。由于检测速度快,样品体积小,并且在便携式微型系统中与PCR相结合,这些设备有望用于SARS-CoV-2检测(Huang et al., 2018)。上述方法的有效性和敏感性有待进一步提高;在改进的情况下,它们可能为唾液检测病毒提供机会,而无需等待时间和复杂的分析基础设施。
Rapid and simple detection of SARS-CoV-2 with point-of-care COVID-19 testing
COVID-19 as a public health concern of the world has spread worldwide and a combination of vari-ous methods including tomography imaging, reverse transcription-polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA) and cell culturing were developed to detect and identify SARS-CoV-2. Due to the absence of specific antiviral agents or vaccines for COVID-19 treat-ment, early detection and identification are vital. An alternative, sensitive, fast point-of-care (POC) detection tool that can be routinely used by health care providers utilizing biological fluids as a specimen before starting an emergency process is desired. Efforts are underway to develop more effective diagnostic and surveillance technologies with loop-mediated isothermal amplification (LAMP) tests, antibody testing and microfluidic RT-PCR devices (Lab-on-a-chip). Point-of-care diagnostics are promising candidates in SARS-Cov-2 detection and encourage scientists to improve their technologies beyond conception. The reverse transcription LAMP (RT-LAMP) method developed for SARS-CoV-2 could detect the virus even in saliva samples in less than an hour (Harapan et al., 2020). Lab-on-a-chip devices contain a small size chip, microchannel, microelectrodes and microheater. Cell ly-sis, DNA extraction and PCR amplification stages could be integrated on these microchips (Sharma et al., 2020). Because of the rapid detection, small volume of the specimen and integration with PCR in a portable tiny system, these devices are promising for SARS-CoV-2 detection (Huang et al., 2018). The validity and sensitivity of all the above-mentioned methods need to be improved for salivary specimen usage; in case of improvement, they might provide an opportunity for salivary detection of the virus without a waiting period and complex analytical infrastructure.
期刊介绍:
Proceedings of the Institute of Mathematics and Mechanics (PIMM), National Academy of Sciences of Azerbaijan is an open access journal that publishes original, high quality research papers in all fields of mathematics. A special attention is paid to the following fields: real and complex analysis, harmonic analysis, functional analysis, approximation theory, differential equations, calculus of variations and optimal control, differential geometry, algebra, number theory, probability theory and mathematical statistics, mathematical physics. PIMM welcomes papers that establish interesting and important new results or solve significant problems. All papers are refereed for correctness and suitability for publication. The journal is published in both print and online versions.