泰国放射性应急准备中双中心和过早染色体凝聚的剂量-反应曲线的建立

Q4 Biochemistry, Genetics and Molecular Biology Genome Integrity Pub Date : 2016-01-01 DOI:10.4103/2041-9414.197165
Benchawan Rungsimaphorn, B. Rerkamnuaychoke, W. Sudprasert
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引用次数: 6

摘要

在泰国首次采用常规生物剂量测定法-双中心染色体测定法(DCA)和染色体过早凝聚法(PCC)进行体外剂量校准曲线,重建受照个体的辐射剂量。健康供者外周血淋巴细胞样本以0.652 Gy/min剂量率辐照137Cs源,DCA技术辐照剂量分别为0.1、0.25、0.5、0.75、1、2、3、4和5 Gy, PCC技术辐照剂量分别为5、10、15、20和25 Gy。血液样本按照国际原子能机构报告中规定的标准程序进行培养和处理,并进行了轻微修改。使用染色体畸变计算软件(CABAS, 2.0版)将至少1000个中期和100个中期的双中心的产率拟合为线性二次方程,而剂量为100个中期的PCC环的产率拟合为线性二次方程,剂量范围为0 ~ 15 Gy。这些曲线将有助于体外剂量重建,并可支持对泰国公共或职业工人过度暴露于辐射和最终发生辐射事故的准备工作。
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Establishment of Dose-response Curves for Dicentrics and Premature Chromosome Condensation for Radiological Emergency Preparedness in Thailand
The in vitro dose calibration curves using conventional biological dosimetry – dicentric chromosome assay (DCA) and premature chromosome condensation (PCC) assay – were performed for the first time in Thailand for reconstruction of radiation dose in the exposed individuals. The peripheral blood lymphocyte samples from healthy donors were irradiated with 137Cs source at a dose rate of 0.652 Gy/min to doses of 0.1, 0.25, 0.5, 0.75, 1, 2, 3, 4, and 5 Gy for DCA technique, and 5, 10, 15, 20, and 25 Gy for PCC technique. The blood samples were cultured and processed following the standard procedure as prescribed in the International Atomic Energy Agency report with slight modifications. The yield of dicentrics with dose from at least 1000 metaphases or 100 dicentrics was fitted to a linear quadratic model using Chromosome Aberration Calculation Software (CABAS, version 2.0) whereas those of PCC rings with dose from 100 rings was fitted to a linear quadratic equation at doses from 0 to 15 Gy. These curves will be useful for in vitro dose reconstruction and can support the preparedness for overexposure to radiation among public or occupational workers and eventual radiological accident in Thailand.
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来源期刊
Genome Integrity
Genome Integrity Biochemistry, Genetics and Molecular Biology-Genetics
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