K. Petrović, L. Riccioni, V. Đorđević, S. Balešević-Tubić, J. Miladinović, M. Ćeran, D. Rajković
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引用次数: 8
摘要
种子腐烂是大豆(Glycine max (L.))的主要病害之一。这对大豆的市场等级产生了负面影响。该疾病主要由长冠散孔虫和其他散孔虫引起。对塞尔维亚伏伊伏丁那省大豆种子健康状况的筛选显示，鉴定为长冠霉的分离株在培养和形态上存在差异。利用内部转录间隔区(ITS1-5.8S-ITS2)和部分翻译延伸因子1-α (EF1-α)的DNA序列，确定了新种。BLAST分析结果表明，该菌株与本文报道的pseudolongicolla (syn. D. novem)同源性为100%，并对其分类进行了讨论。致病性试验表明，长叶霉和假长叶霉对大豆茎和种子都有很高的致病性，造成100%的茎萎蔫和82%以上的种子腐烂。
Diaporthe pseudolongicolla: The new pathogen on soybean seed in Serbia
Seed decay is one of the most important diseases of soybean (Glycine max (L.) Merr.) that has a negative impact on the market grade of soybeans. The disease is mainly caused by Diaporthe longicolla, along with other Diaporthe species. Screening of soybean seeds health status in Vojvodina Province, Serbia, showed cultural and morphological variability among isolates identified as D. longicolla. With the use of DNA sequences of internal transcribed spacer (ITS1-5.8S-ITS2) region and partial translation elongation factor 1-alpha (EF1-α), the new species was determined. BLAST analysis showed 100% identity with D. pseudolongicolla (syn. D. novem) that was described in this study and its taxonomic revision is discussed. Pathogenicity trial showed that both species, D. longicolla and D. pseudolongicolla, are highly pathogenic on soybean stem and seed, causing 100% of stem wilting and more than 82% of seed decay.
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