塞尔维亚红花黄瓜花叶病毒的存在及其分子特征

Q4 Biochemistry, Genetics and Molecular Biology Ratarstvo i Povrtarstvo Pub Date : 2020-01-01 DOI:10.5937/ratpov57-25745
D. Milošević, M. Ignjatov, A. Marjanović-Jeromela, Z. Nikolić, G. Tamindžić, D. Miljaković, I. Stanković
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引用次数: 1

摘要

红花(Carthamus tinctorius L.)是菊科重要的油料作物。2015年,在塞尔维亚Srbobran地区(South Backa区)采集了46份红花样品,采用商业双抗体夹心(DAS)-ELISA试剂盒检测黄瓜花叶病毒(CMV)、苜蓿花叶病毒(AMV)和生菜花叶病毒(LMV)的存在情况。在收集的样本中进行了CMV和AMV两种病毒的血清学检测。经分析的样本均未发现LMV呈阳性。通过机械传播对藜麦藜、苋菜藜、烟叶藜、曼陀罗藜和橘色藜等植物的检测,进一步证实了巨细胞病毒的存在,证实了该疾病的传染性。利用CMVCPfwd/CMVCPrev特异性引物扩增CMV RNA 3的整个外壳蛋白(CP)基因和部分3′和5′- utr,在所有样品中扩增一个871 bp的片段进行CMV分子检测。对分离株290Saff和294Saff的RT-PCR产物(分别为MH577791和MH577792)进行测序,并与GenBank中CMV序列进行比较。基于CP基因序列的系统发育分析表明,所选菌株可分为IA、IB和II三个亚群。在红花中发现的塞尔维亚CMV分离株属于II亚群。据我们所知,这是塞尔维亚红花CMV感染的第一份报告,这种病毒有可能对红花生产造成重大损害,并对塞尔维亚种植的其他经济作物构成威胁。
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Presence and molecular characterization of cucumber mosaic virus on safflower in Serbia
Safflower (Carthamus tinctorius L.) is an important oilseed crop belonging to the family Asteraceae. A total of 46 safflower samples were collected from Srbobran locality (South Backa District) in Serbia in 2015 and analysed for the presence of cucumber mosaic virus (CMV), alfalfa mosaic virus (AMV), and lettuce mosaic virus (LMV), using commercial double-antibody sandwich (DAS)-ELISA kits. Both viruses, CMV and AMV, were detected serologically in the collected samples. None of the analysed samples was found to be positive for LMV. The presence of CMV was further confirmed by mechanical transmission to test the plants including Chenopodium quinoa, C. amaranticolor, Nicotiana glutinosa, and Datura stramonium as well as C. tinctorius, confirming the infectious nature of the disease. Molecular detection of CMV was performed by amplification of a 871 bp fragment in all the tested samples, using the specific primers CMVCPfwd/CMVCPrev that amplify the entire coat protein (CP) gene and part of 3'and 5'-UTRs of CMV RNA 3. The RT-PCR products derived from the isolates 290Saff and 294Saff were sequenced (MH577791 and MH577792, respectively) and compared with the CMV sequences available in GenBank. Phylogenetic analysis based on CP gene sequences showed clustering of the selected isolates into three subgroups: IA, IB and II. Serbian CMV isolates found in safflower belong to subgroup II. To our knowledge, this is the first report on CMV infection of safflower in Serbia, which has the potential to cause substantial damage to safflower production and pose a threat to other economic crops grown in Serbia.
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来源期刊
Ratarstvo i Povrtarstvo
Ratarstvo i Povrtarstvo Agricultural and Biological Sciences-Soil Science
CiteScore
0.80
自引率
0.00%
发文量
4
审稿时长
4 weeks
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