Rakesh Ranjan, S. D. Narnaware, Dinesh Harsh, N. Pareek, A. Ranjan, Jeeshan Nabi, A. Sahoo
{"title":"从印度单峰骆驼干酪性淋巴结炎中分离出一株硝酸还原酶阳性假结核棒状杆菌","authors":"Rakesh Ranjan, S. D. Narnaware, Dinesh Harsh, N. Pareek, A. Ranjan, Jeeshan Nabi, A. Sahoo","doi":"10.5958/2277-8934.2023.00027.9","DOIUrl":null,"url":null,"abstract":"The present study reports isolation of a nitrate reductase positive strain of Corynebacterium pseudotuberculosis from caseous lymphadenitis in a dromedary camel housed in an organised dromedary camel farm located at Bikaner, Rajasthan, India. The bacteria were identified on the basis of biochemical properties as well as PCR based amplification of 16s rDNA, rpoB, plD and narG genes. Phylogenetic analysis using partial sequences of 16s rDNA and rpoB genes revealed its resemblance to equine strains. It was hypothesised that the organism might have originated from the equine herd located in the vicinity of the camel farm. The isolated strain was resistant to penicillin, oxacillin, cloxacillin, amoxicillin/ sulbactam and methicillin but sensitive to amikacin, cefoxitin, ceftriaxone, chloramphenicol, ciprofloxacin, doxycycline hydrochloride","PeriodicalId":15097,"journal":{"name":"Journal of Camel Practice and Research","volume":"1 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Isolation and Characterisation of a Nitrate Reductase Positive Corynebacterium pseudotuberculosis Strain from Caseous Lymphadenitis in a Dromedary Camel (Camelus dromedarius) from India\",\"authors\":\"Rakesh Ranjan, S. D. Narnaware, Dinesh Harsh, N. Pareek, A. Ranjan, Jeeshan Nabi, A. Sahoo\",\"doi\":\"10.5958/2277-8934.2023.00027.9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The present study reports isolation of a nitrate reductase positive strain of Corynebacterium pseudotuberculosis from caseous lymphadenitis in a dromedary camel housed in an organised dromedary camel farm located at Bikaner, Rajasthan, India. The bacteria were identified on the basis of biochemical properties as well as PCR based amplification of 16s rDNA, rpoB, plD and narG genes. Phylogenetic analysis using partial sequences of 16s rDNA and rpoB genes revealed its resemblance to equine strains. It was hypothesised that the organism might have originated from the equine herd located in the vicinity of the camel farm. The isolated strain was resistant to penicillin, oxacillin, cloxacillin, amoxicillin/ sulbactam and methicillin but sensitive to amikacin, cefoxitin, ceftriaxone, chloramphenicol, ciprofloxacin, doxycycline hydrochloride\",\"PeriodicalId\":15097,\"journal\":{\"name\":\"Journal of Camel Practice and Research\",\"volume\":\"1 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Camel Practice and Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5958/2277-8934.2023.00027.9\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Camel Practice and Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5958/2277-8934.2023.00027.9","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Isolation and Characterisation of a Nitrate Reductase Positive Corynebacterium pseudotuberculosis Strain from Caseous Lymphadenitis in a Dromedary Camel (Camelus dromedarius) from India
The present study reports isolation of a nitrate reductase positive strain of Corynebacterium pseudotuberculosis from caseous lymphadenitis in a dromedary camel housed in an organised dromedary camel farm located at Bikaner, Rajasthan, India. The bacteria were identified on the basis of biochemical properties as well as PCR based amplification of 16s rDNA, rpoB, plD and narG genes. Phylogenetic analysis using partial sequences of 16s rDNA and rpoB genes revealed its resemblance to equine strains. It was hypothesised that the organism might have originated from the equine herd located in the vicinity of the camel farm. The isolated strain was resistant to penicillin, oxacillin, cloxacillin, amoxicillin/ sulbactam and methicillin but sensitive to amikacin, cefoxitin, ceftriaxone, chloramphenicol, ciprofloxacin, doxycycline hydrochloride
期刊介绍:
JCPR is an exclusive journal which brings out the manuscripts based on New World and Old World camelids. This journal provided a very good platform to publish camelid literature with a view to find the missing links of research and to update the camelids practitioners and researchers with latest research.