{"title":"不同种植材料培养的大鼠成骨细胞中钙、磷酸盐和碱性磷酸酶活性的体外水平","authors":"F. Shaama","doi":"10.7392/OPENACCESS.70081966","DOIUrl":null,"url":null,"abstract":"Osteoblast cells from newborn Sprague-Dawley rats were harvested from calvaria. Cells were cultivated in the presence of Cobalt-Chrome (Co-Cr), cpTitanium (Ti), polymethylmethacrylate (PMMA), and wood in artificial media in humidified atmosphere at 37 0 C, 5% CO 2 . Cellular function was monitored over two weeks. Results indicated materials were not significant for Ca 2+ , and PO 4 2- but the time and interaction was significant (p<0.05). For ALP, materials, time and interaction was significant (p<0.05). Wood produced the highest activity for ALP. Electron microscopic studies captured cells growing over all materials and the presence of Ca 2+ in wood samples only.","PeriodicalId":35436,"journal":{"name":"Trends in Biomaterials and Artificial Organs","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2013-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"In Vitro Levels of Calcium, Phosphate and Alkaline Phosphatase Activity in Media of Rat Osteoblast Grown in the Presence of Various Implant Materials\",\"authors\":\"F. Shaama\",\"doi\":\"10.7392/OPENACCESS.70081966\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Osteoblast cells from newborn Sprague-Dawley rats were harvested from calvaria. Cells were cultivated in the presence of Cobalt-Chrome (Co-Cr), cpTitanium (Ti), polymethylmethacrylate (PMMA), and wood in artificial media in humidified atmosphere at 37 0 C, 5% CO 2 . Cellular function was monitored over two weeks. Results indicated materials were not significant for Ca 2+ , and PO 4 2- but the time and interaction was significant (p<0.05). For ALP, materials, time and interaction was significant (p<0.05). Wood produced the highest activity for ALP. Electron microscopic studies captured cells growing over all materials and the presence of Ca 2+ in wood samples only.\",\"PeriodicalId\":35436,\"journal\":{\"name\":\"Trends in Biomaterials and Artificial Organs\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2013-05-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Trends in Biomaterials and Artificial Organs\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.7392/OPENACCESS.70081966\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Trends in Biomaterials and Artificial Organs","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.7392/OPENACCESS.70081966","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}
In Vitro Levels of Calcium, Phosphate and Alkaline Phosphatase Activity in Media of Rat Osteoblast Grown in the Presence of Various Implant Materials
Osteoblast cells from newborn Sprague-Dawley rats were harvested from calvaria. Cells were cultivated in the presence of Cobalt-Chrome (Co-Cr), cpTitanium (Ti), polymethylmethacrylate (PMMA), and wood in artificial media in humidified atmosphere at 37 0 C, 5% CO 2 . Cellular function was monitored over two weeks. Results indicated materials were not significant for Ca 2+ , and PO 4 2- but the time and interaction was significant (p<0.05). For ALP, materials, time and interaction was significant (p<0.05). Wood produced the highest activity for ALP. Electron microscopic studies captured cells growing over all materials and the presence of Ca 2+ in wood samples only.
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