在正常表皮黑素细胞中,细胞球蛋白作为黑素生成的氧化还原调节剂发挥作用。

IF 3.9 3区 医学 Q2 CELL BIOLOGY Pigment Cell & Melanoma Research Pub Date : 2023-11-02 DOI:10.1111/pcmr.13146
Yo Tanaka, Misako Sato-Matsubara, Daisuke Tsuruta, Hiroshi Tanaka, Chiho Kadono, Koji Sugawara, Norifumi Kawada, Kazumasa Wakamatsu, Shosuke Ito, Katsutoshi Yoshizato
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引用次数: 0

摘要

表皮黑色素细胞持续暴露于阳光诱导的活性氧(ROS)和黑色素合成过程中产生的氧化应激。因此,他们开发了维持正常氧化还原稳态的机制。细胞球蛋白(CYGB)是一种广泛表达的细胞内铁六配位球蛋白,具有抗氧化活性,并通过其作为过氧化物酶和一氧化氮(NO)双加氧酶的活性调节哺乳动物细胞的氧化还原状态。我们假设CYGB在黑色素生成过程中作为一种调节因子发挥作用,将氧化应激维持在生理水平内。这是通过使用形态学和分子生物学标准用CYGB的敲除(KD)表征正常人黑素细胞来进行检测的。在黑色素生成的晚期,CYGB-KD细胞比对照细胞更大,有更多的树突,并产生更多的黑色素颗粒。CYGB-KD黑色素细胞中主要黑色素生成相关基因和蛋白质的表达水平高于野生型(WT)细胞。正如预期的那样,CYGB-KD黑素细胞比WT细胞产生更多的ROS和NO。总之,CYGB通过控制细胞内ROS和NO的水平,在生理上有助于维持正常黑素细胞黑素生成活性的氧化还原稳态。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Cytoglobin functions as a redox regulator of melanogenesis in normal epidermal melanocytes

Epidermal melanocytes are continuously exposed to sunlight-induced reactive oxygen species (ROS) and oxidative stress generated during the synthesis of melanin. Therefore, they have developed mechanisms that maintain normal redox homeostasis. Cytoglobin (CYGB), a ubiquitously expressed intracellular iron hexacoordinated globin, exhibits antioxidant activity and regulates the redox state of mammalian cells through its activities as peroxidase and nitric oxide (NO) dioxygenase. We postulated that CYGB functions in the melanogenic process as a regulator that maintains oxidative stress within a physiological level. This was examined by characterizing normal human melanocytes with the knockdown (KD) of CYGB using morphological and molecular biological criteria. CYGB-KD cells were larger, had more dendrites, and generated more melanin granules in the advanced stages of melanogenesis than control cells. The expression levels of major melanogenesis-associated genes and proteins were higher in CYGB-KD melanocytes than in wild type (WT) cells. As expected, CYGB-KD melanocytes generated more ROS and NO than WT cells. In conclusion, CYGB physiologically contributes to maintaining redox homeostasis in the melanogenic activity of normal melanocytes by controlling the intracellular levels of ROS and NO.

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来源期刊
Pigment Cell & Melanoma Research
Pigment Cell & Melanoma Research 医学-皮肤病学
CiteScore
8.90
自引率
2.30%
发文量
54
审稿时长
6-12 weeks
期刊介绍: Pigment Cell & Melanoma Researchpublishes manuscripts on all aspects of pigment cells including development, cell and molecular biology, genetics, diseases of pigment cells including melanoma. Papers that provide insights into the causes and progression of melanoma including the process of metastasis and invasion, proliferation, senescence, apoptosis or gene regulation are especially welcome, as are papers that use the melanocyte system to answer questions of general biological relevance. Papers that are purely descriptive or make only minor advances to our knowledge of pigment cells or melanoma in particular are not suitable for this journal. Keywords Pigment Cell & Melanoma Research, cell biology, melatonin, biochemistry, chemistry, comparative biology, dermatology, developmental biology, genetics, hormones, intracellular signalling, melanoma, molecular biology, ocular and extracutaneous melanin, pharmacology, photobiology, physics, pigmentary disorders
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