海马胰岛素受体通过兴奋/抑制平衡调节情景和空间记忆。

IF 3.9 4区 医学 Q2 NEUROSCIENCES ASN NEURO Pub Date : 2023-01-01 DOI:10.1177/17590914231206657
Cai-Yan Xue, Tian Gao, E Mao, Zhen-Zhen Kou, Ling Dong, Feng Gao
{"title":"海马胰岛素受体通过兴奋/抑制平衡调节情景和空间记忆。","authors":"Cai-Yan Xue, Tian Gao, E Mao, Zhen-Zhen Kou, Ling Dong, Feng Gao","doi":"10.1177/17590914231206657","DOIUrl":null,"url":null,"abstract":"<p><p>It is well known that the hippocampus is a vital brain region playing a key role in both episodic and spatial memory. Insulin receptors (InsRs) are densely distributed in the hippocampus and are important for its function. However, the effects of InsRs on the function of the specific hippocampal cell types remain elusive. In this study, hippocampal InsRs knockout mice had impaired episodic and spatial memory. GABAergic neurons and glutamatergic neurons in the hippocampus are involved in the balance between excitatory and inhibitory (E/I) states and participate in the processes of episodic and spatial memory. InsRs are located mainly at excitatory neurons in the hippocampus, whereas 8.5% of InsRs are glutamic acid decarboxylase 2 (GAD2)::Ai9-positive (GABAergic) neurons. Next, we constructed a transgenic mouse system in which InsR expression was deleted from GABAergic (glutamate decarboxylase 2::InsR<sup>fl/fl</sup>, GAD2<sup>Cre</sup>::InsR<sup>fl/fl</sup>) or glutamatergic neurons (vesicular glutamate transporter 2::InsR<sup>fl/fl</sup>,Vglut2<sup>Cre</sup>::InsR<sup>fl/fl</sup>). Our results showed that in comparison to the InsR<sup>fl/fl</sup> mice, both episodic and spatial memory were lower in GAD2<sup>Cre</sup>::InsR<sup>fl/fl</sup> and Vglut2<sup>Cre</sup>::InsR<sup>fl/fl</sup>. In addition, both GAD2<sup>Cre</sup>::InsR<sup>fl/fl</sup> and Vglut2<sup>Cre</sup>::InsR<sup>fl/fl</sup> were associated with more anxiety and lower glucose tolerance. These findings reveal that hippocampal InsRs might be crucial for episodic and spatial memory through E/I balance hippocampal regulation.</p>","PeriodicalId":8616,"journal":{"name":"ASN NEURO","volume":"15 ","pages":"17590914231206657"},"PeriodicalIF":3.9000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10621302/pdf/","citationCount":"0","resultStr":"{\"title\":\"Hippocampus Insulin Receptors Regulate Episodic and Spatial Memory Through Excitatory/Inhibitory Balance.\",\"authors\":\"Cai-Yan Xue, Tian Gao, E Mao, Zhen-Zhen Kou, Ling Dong, Feng Gao\",\"doi\":\"10.1177/17590914231206657\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>It is well known that the hippocampus is a vital brain region playing a key role in both episodic and spatial memory. Insulin receptors (InsRs) are densely distributed in the hippocampus and are important for its function. However, the effects of InsRs on the function of the specific hippocampal cell types remain elusive. In this study, hippocampal InsRs knockout mice had impaired episodic and spatial memory. GABAergic neurons and glutamatergic neurons in the hippocampus are involved in the balance between excitatory and inhibitory (E/I) states and participate in the processes of episodic and spatial memory. InsRs are located mainly at excitatory neurons in the hippocampus, whereas 8.5% of InsRs are glutamic acid decarboxylase 2 (GAD2)::Ai9-positive (GABAergic) neurons. Next, we constructed a transgenic mouse system in which InsR expression was deleted from GABAergic (glutamate decarboxylase 2::InsR<sup>fl/fl</sup>, GAD2<sup>Cre</sup>::InsR<sup>fl/fl</sup>) or glutamatergic neurons (vesicular glutamate transporter 2::InsR<sup>fl/fl</sup>,Vglut2<sup>Cre</sup>::InsR<sup>fl/fl</sup>). Our results showed that in comparison to the InsR<sup>fl/fl</sup> mice, both episodic and spatial memory were lower in GAD2<sup>Cre</sup>::InsR<sup>fl/fl</sup> and Vglut2<sup>Cre</sup>::InsR<sup>fl/fl</sup>. In addition, both GAD2<sup>Cre</sup>::InsR<sup>fl/fl</sup> and Vglut2<sup>Cre</sup>::InsR<sup>fl/fl</sup> were associated with more anxiety and lower glucose tolerance. These findings reveal that hippocampal InsRs might be crucial for episodic and spatial memory through E/I balance hippocampal regulation.</p>\",\"PeriodicalId\":8616,\"journal\":{\"name\":\"ASN NEURO\",\"volume\":\"15 \",\"pages\":\"17590914231206657\"},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10621302/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ASN NEURO\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1177/17590914231206657\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"NEUROSCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ASN NEURO","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1177/17590914231206657","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

众所周知,海马体是大脑中至关重要的区域,在情景记忆和空间记忆中都发挥着关键作用。胰岛素受体(InsRs)密集分布在海马体中,对其功能很重要。然而,InsRs对特定海马细胞类型功能的影响仍然难以捉摸。在这项研究中,海马InsRs敲除小鼠的情景记忆和空间记忆受损。海马中的GABA能神经元和谷氨酸能神经元参与兴奋和抑制(E/I)状态之间的平衡,并参与情景记忆和空间记忆的过程。InsRs主要位于海马的兴奋性神经元,而8.5%的InsRs是谷氨酸脱羧酶2(GAD2)::Ai9阳性(GABA能)神经元。接下来,我们构建了一个转基因小鼠系统,其中InsR表达从GABA能神经元(谷氨酸脱羧酶2::InsRfl/fl,GAD2Cr::InsRfl/fl)或谷氨酸能神经元(囊泡谷氨酸转运体2::InsRfl/fl,Vglut2Cr::InsRfl/fl)中缺失。我们的研究结果表明,与InsRfl/fl小鼠相比,GAD2Cr::InsRfl/fl和Vglut2Cr::Insirfl/fl小鼠的情景记忆和空间记忆都较低。此外,GAD2Cr::InsRfl/fl和Vglut2Cr::Insirfl/fl都与更多的焦虑和更低的糖耐量有关。这些发现表明,海马InsRs可能通过E/I平衡海马调节对情景记忆和空间记忆至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

摘要图片

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Hippocampus Insulin Receptors Regulate Episodic and Spatial Memory Through Excitatory/Inhibitory Balance.

It is well known that the hippocampus is a vital brain region playing a key role in both episodic and spatial memory. Insulin receptors (InsRs) are densely distributed in the hippocampus and are important for its function. However, the effects of InsRs on the function of the specific hippocampal cell types remain elusive. In this study, hippocampal InsRs knockout mice had impaired episodic and spatial memory. GABAergic neurons and glutamatergic neurons in the hippocampus are involved in the balance between excitatory and inhibitory (E/I) states and participate in the processes of episodic and spatial memory. InsRs are located mainly at excitatory neurons in the hippocampus, whereas 8.5% of InsRs are glutamic acid decarboxylase 2 (GAD2)::Ai9-positive (GABAergic) neurons. Next, we constructed a transgenic mouse system in which InsR expression was deleted from GABAergic (glutamate decarboxylase 2::InsRfl/fl, GAD2Cre::InsRfl/fl) or glutamatergic neurons (vesicular glutamate transporter 2::InsRfl/fl,Vglut2Cre::InsRfl/fl). Our results showed that in comparison to the InsRfl/fl mice, both episodic and spatial memory were lower in GAD2Cre::InsRfl/fl and Vglut2Cre::InsRfl/fl. In addition, both GAD2Cre::InsRfl/fl and Vglut2Cre::InsRfl/fl were associated with more anxiety and lower glucose tolerance. These findings reveal that hippocampal InsRs might be crucial for episodic and spatial memory through E/I balance hippocampal regulation.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
ASN NEURO
ASN NEURO NEUROSCIENCES-
CiteScore
7.70
自引率
4.30%
发文量
35
审稿时长
>12 weeks
期刊介绍: ASN NEURO is an open access, peer-reviewed journal uniquely positioned to provide investigators with the most recent advances across the breadth of the cellular and molecular neurosciences. The official journal of the American Society for Neurochemistry, ASN NEURO is dedicated to the promotion, support, and facilitation of communication among cellular and molecular neuroscientists of all specializations.
期刊最新文献
Cellular Mechanisms of Cognitive Enhancement: The In Vivo Modulation of the Firing Activity and the Responsiveness of Rat Hippocampal Neurons by Memantine and Alpha7 Nicotinic Acetylcholine Receptor Ligands. Diverse Responses of Oligodendrocytes to Different FGF-Family Members: Uncoupling Structure-Function Relationship Within FGF Subfamilies. Pannexin1 Mediates Early-Life Seizure-Induced Social Behavior Deficits. Reduced Expression of Oligodendrocyte Linage-Enriched Transcripts During the Endoplasmic Reticulum Stress/Integrated Stress Response. Steroidogenic Factor-1 Regulation of Dorsomedial Ventromedial Hypothalamic Nucleus Ghrh Neuron Transmitter Marker and Estrogen Receptor Gene Expression in Male Rat.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1