以脊髓灰质炎疫苗为例,以假病毒颗粒为基础研制新型疫苗制剂的前景。

Q3 Biochemistry, Genetics and Molecular Biology Biomeditsinskaya khimiya Pub Date : 2023-11-01 DOI:10.18097/PBMC20236905253
D D Zhdanov, Yu Yu Ivin, A N Shishparenok, S V Kraevskiy, S L Kanashenko, L E Agafonova, V V Shumyantseva, O V Gnedenko, A N Pinyaeva, A A Kovpak, A A Ishmukhametov, A I Archakov
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引用次数: 0

摘要

传统的抗病毒疫苗目前是通过化学灭活病毒来制造的,通常使用甲醛或β-丙内酯。这些方法不是最佳的,因为它们对灭活颗粒的抗原决定簇的安全性产生负面影响,并且需要额外的纯化阶段。最有前途的疫苗生产平台是基于假病毒,即完全保留外壳(衣壳),但由于基因组的破坏而失去繁殖能力的病毒。用电子束照射病毒是制造假病毒颗粒的最佳方式。在这篇综述中,以脊髓灰质炎病毒为例,介绍了在疫苗制备过程中可用于在功能和结构上表征假病毒颗粒的主要算法。这些算法就是分析基因组的破坏程度和共免疫性。考虑了脊髓灰质炎病毒的结构及其灭活方法。为假病毒的功能表征,提出了评估残留传染性和免疫原性的方法。基因组完整性分析方法、原子力和电子显微镜、表面等离子体共振和生物电化学方法对假病毒颗粒的结构表征至关重要。
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Perspectives for the creation of a new type of vaccine preparations based on pseudovirus particles using polio vaccine as an example.

Traditional antiviral vaccines are currently created by inactivating the virus chemically, most often using formaldehyde or β-propiolactone. These approaches are not optimal since they negatively affect the safety of the antigenic determinants of the inactivated particles and require additional purification stages. The most promising platforms for creating vaccines are based on pseudoviruses, i.e., viruses that have completely preserved the outer shell (capsid), while losing the ability to reproduce owing to the destruction of the genome. The irradiation of viruses with electron beam is the optimal way to create pseudoviral particles. In this review, with the example of the poliovirus, the main algorithms that can be applied to characterize pseudoviral particles functionally and structurally in the process of creating a vaccine preparation are presented. These algorithms are, namely, the analysis of the degree of genome destruction and coimmunogenicity. The structure of the poliovirus and methods of its inactivation are considered. Methods for assessing residual infectivity and immunogenicity are proposed for the functional characterization of pseudoviruses. Genome integrity analysis approaches, atomic force and electron microscopy, surface plasmon resonance, and bioelectrochemical methods are crucial to structural characterization of the pseudovirus particles.

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来源期刊
Biomeditsinskaya khimiya
Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
1.30
自引率
0.00%
发文量
49
期刊介绍: The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).
期刊最新文献
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